000282307 001__ 282307 000282307 005__ 20251117153804.0 000282307 037__ $$aDZNE-2025-01277 000282307 1001_ $$0P:(DE-2719)9001111$$aAkoury, Elias$$b0 000282307 245__ $$aMolecular Mechanisms of Tau Protein Aggregation Inhibition 000282307 260__ $$c2013 000282307 300__ $$a240 p. 000282307 3367_ $$2DataCite$$aOutput Types/Dissertation 000282307 3367_ $$2ORCID$$aDISSERTATION 000282307 3367_ $$2BibTeX$$aPHDTHESIS 000282307 3367_ $$02$$2EndNote$$aThesis 000282307 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1763390261_17166 000282307 3367_ $$2DRIVER$$adoctoralThesis 000282307 502__ $$aDissertation, Georg-August-Universität Göttingen, 2013$$bDissertation$$cGeorg-August-Universität Göttingen$$d2013$$o2013-09-30 000282307 520__ $$aOf all neurodegenerative diseases, Alzheimer´s Disease is the most widespread dementia syndrome, exhibiting progressive memory loss and intellectual abilities. One of the pathological hallmarks of this disease is associated to the presence of abundant intracellular deposits of the Tau protein. Accumulation of these stable species is a multistep process that involves the formation of various transients. Understanding this key step may eventually enable to obstruct aggregation. Tau protein is an intrinsically disordered protein, abundant in neuronal axons where it promotes and stabilizes microtubule assembly. Chapter 1 discusses the physiological function and pathological consequences of this protein and sheds light on the current Tau-based research implemented in therapeutic strategies. This chapter also reviews the identification of inhibitors of Tau aggregation as potential disease-modifying drugs; with a detailed discussion of two organic compounds we have recently screened, Phthalocyanine Tetrasulfonate and Phenothiazine Methylene Blue. The same chapter then introduces the Heat shock proteins and their role in Tau clearance. The chapters that follow report our research investigation of aggregation inhibitors and chaperonic partners of Tau where we used an integrated approach to detect possible assemblies of pathogenic conformational transitions. Chapter 2 represents the Phthalocyanine tetrasulfonate study and its inhibition of Tau filament formation by conformational modulation. Chapter 3 investigates the mechanistic basis of the phenothiazine-driven inhibition of Tau aggregation. Chapter 4, examines how the imbalance of Hsp70 family variants fosters Tau accumulation. As a final point, the significance of the biophysical techniques incorporated in the three published manuscripts are briefly discussed in the last part of Chapter 1. 000282307 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0 000282307 588__ $$aDataset connected to DataCite 000282307 8564_ $$uhttp://dx.doi.org/10.53846/goediss-4145$$yRestricted 000282307 8564_ $$uhttps://pub.dzne.de/record/282307/files/DZNE-2025-01277.pdf$$yRestricted 000282307 8564_ $$uhttps://pub.dzne.de/record/282307/files/DZNE-2025-01277.pdf?subformat=pdfa$$xpdfa$$yRestricted 000282307 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001111$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE 000282307 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0 000282307 9201_ $$0I:(DE-2719)1410001$$kAG Zweckstetter$$lTranslational Structural Biology$$x0 000282307 980__ $$aphd 000282307 980__ $$aEDITORS 000282307 980__ $$aVDBINPRINT 000282307 980__ $$aI:(DE-2719)1410001 000282307 980__ $$aUNRESTRICTED