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000282545 005__ 20251202110826.0
000282545 020__ $$a978-1-0716-4806-3 (print)
000282545 020__ $$a978-1-0716-4807-0 (electronic)
000282545 0247_ $$2doi$$a10.1007/978-1-0716-4807-0_12
000282545 0247_ $$2ISSN$$a1064-3745
000282545 0247_ $$2ISSN$$a1940-6029
000282545 037__ $$aDZNE-2025-01308
000282545 041__ $$aEnglish
000282545 082__ $$a570
000282545 1001_ $$aKobayashi, Kazuto$$b0$$eEditor
000282545 245__ $$aApplication of Lentivirus Vectors to Adult Neurogenesis and Manipulation of Neural Stem Cells
000282545 260__ $$aNew York, NY$$bSpringer US$$c2026
000282545 29510 $$aLentiviral Vectors / Kobayashi, Kazuto (Editor) ; New York, NY : Springer US, 2026, Chapter 12 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-4806-3=978-1-0716-4807-0 ; doi:10.1007/978-1-0716-4807-0
000282545 300__ $$a141 - 151
000282545 3367_ $$2ORCID$$aBOOK_CHAPTER
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000282545 3367_ $$2BibTeX$$aINBOOK
000282545 3367_ $$2DataCite$$aOutput Types/Book chapter
000282545 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1764670035_28230
000282545 4900_ $$aMethods in Molecular Biology$$v2974
000282545 520__ $$aLentivirus vectors are capable of efficient gene transfer to nondividing cells, in addition to dividing cells. Therefore, lentivirus vectors can also be used for gene transfer into dormant stem cells in the postnatal and adult brains of experimental animals, many of which have ceased cell division. For example, it is known that neural stem cells (NSCs) are present around the lateral ventricle and in the dentate gyrus of the hippocampus in the postnatal and adult brain and continue to give rise to neurons. However, the majority of these NSCs are considered to be quiescent, and lentivirus vectors are an important tool to enable gene transfer to dormant stem cells. Lentivirus vectors are also capable of multiple infection of the same cells, making them an important option for experiments requiring high gene expression levels. By selecting transduced cells after infection with high titer lentivirus vectors and repeating infection, genes that are difficult to express or gene sequences that require high copy number can be introduced into cells with high efficiency. In this chapter, we present examples of gene transfer to NSCs localized at the hippocampal dentate gyrus in mice using lentivirus vectors and establishment of stable transgenic NSC lines by multiple infections.
000282545 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000282545 588__ $$aDataset connected to CrossRef Book Series, Journals: pub.dzne.de
000282545 650_7 $$2Other$$aHippocampus
000282545 650_7 $$2Other$$aLentivirus vector
000282545 650_7 $$2Other$$aNeural stem cells
000282545 650_7 $$2Other$$aOptogenetics
000282545 650_7 $$2Other$$aQuiescent cells
000282545 650_7 $$2Other$$abHLH transcription factor
000282545 650_2 $$2MeSH$$aLentivirus: genetics
000282545 650_2 $$2MeSH$$aNeural Stem Cells: cytology
000282545 650_2 $$2MeSH$$aNeural Stem Cells: metabolism
000282545 650_2 $$2MeSH$$aGenetic Vectors: genetics
000282545 650_2 $$2MeSH$$aAnimals
000282545 650_2 $$2MeSH$$aNeurogenesis: genetics
000282545 650_2 $$2MeSH$$aMice
000282545 650_2 $$2MeSH$$aTransduction, Genetic
000282545 650_2 $$2MeSH$$aDentate Gyrus: cytology
000282545 650_2 $$2MeSH$$aDentate Gyrus: metabolism
000282545 650_2 $$2MeSH$$aGene Transfer Techniques
000282545 7001_ $$aHirai, Hirokazu$$b1$$eEditor
000282545 7001_ $$aHioki, Hiroyuki$$b2$$eEditor
000282545 7001_ $$aImayoshi, Itaru$$b3
000282545 7001_ $$aYamada, Mayumi$$b4
000282545 7001_ $$aNagasaki, Shinji C.$$b5
000282545 7001_ $$aFukuda, Tomonori D.$$b6
000282545 7001_ $$aNagano, Ikumi$$b7
000282545 7001_ $$0P:(DE-2719)2814117$$aToda, Tomohisa$$b8$$eLast author$$udzne
000282545 773__ $$a10.1007/978-1-0716-4807-0_12
000282545 8564_ $$uhttps://pub.dzne.de/record/282545/files/DZNE-2025-01308_Restricted.pdf
000282545 8564_ $$uhttps://pub.dzne.de/record/282545/files/DZNE-2025-01308_Restricted.pdf?subformat=pdfa$$xpdfa
000282545 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2814117$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b8$$kDZNE
000282545 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000282545 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-19
000282545 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-19
000282545 9201_ $$0I:(DE-2719)1710014$$kAG Toda$$lNuclear Architecture in Neural Plasticity and Aging$$x0
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