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@INBOOK{Kobayashi:282545,
      author       = {Imayoshi, Itaru and Yamada, Mayumi and Nagasaki, Shinji C.
                      and Fukuda, Tomonori D. and Nagano, Ikumi and Toda,
                      Tomohisa},
      editor       = {Kobayashi, Kazuto and Hirai, Hirokazu and Hioki, Hiroyuki},
      title        = {{A}pplication of {L}entivirus {V}ectors to {A}dult
                      {N}eurogenesis and {M}anipulation of {N}eural {S}tem
                      {C}ells},
      volume       = {2974},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {DZNE-2025-01308},
      isbn         = {978-1-0716-4806-3 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {141 - 151},
      year         = {2026},
      comment      = {Lentiviral Vectors / Kobayashi, Kazuto (Editor) ; New York,
                      NY : Springer US, 2026, Chapter 12 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-0716-4806-3=978-1-0716-4807-0 ;
                      doi:10.1007/978-1-0716-4807-0},
      booktitle     = {Lentiviral Vectors / Kobayashi, Kazuto
                       (Editor) ; New York, NY : Springer US,
                       2026, Chapter 12 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-4806-3=978-1-0716-4807-0 ;
                       doi:10.1007/978-1-0716-4807-0},
      abstract     = {Lentivirus vectors are capable of efficient gene transfer
                      to nondividing cells, in addition to dividing cells.
                      Therefore, lentivirus vectors can also be used for gene
                      transfer into dormant stem cells in the postnatal and adult
                      brains of experimental animals, many of which have ceased
                      cell division. For example, it is known that neural stem
                      cells (NSCs) are present around the lateral ventricle and in
                      the dentate gyrus of the hippocampus in the postnatal and
                      adult brain and continue to give rise to neurons. However,
                      the majority of these NSCs are considered to be quiescent,
                      and lentivirus vectors are an important tool to enable gene
                      transfer to dormant stem cells. Lentivirus vectors are also
                      capable of multiple infection of the same cells, making them
                      an important option for experiments requiring high gene
                      expression levels. By selecting transduced cells after
                      infection with high titer lentivirus vectors and repeating
                      infection, genes that are difficult to express or gene
                      sequences that require high copy number can be introduced
                      into cells with high efficiency. In this chapter, we present
                      examples of gene transfer to NSCs localized at the
                      hippocampal dentate gyrus in mice using lentivirus vectors
                      and establishment of stable transgenic NSC lines by multiple
                      infections.},
      keywords     = {Lentivirus: genetics / Neural Stem Cells: cytology / Neural
                      Stem Cells: metabolism / Genetic Vectors: genetics / Animals
                      / Neurogenesis: genetics / Mice / Transduction, Genetic /
                      Dentate Gyrus: cytology / Dentate Gyrus: metabolism / Gene
                      Transfer Techniques / Hippocampus (Other) / Lentivirus
                      vector (Other) / Neural stem cells (Other) / Optogenetics
                      (Other) / Quiescent cells (Other) / bHLH transcription
                      factor (Other)},
      cin          = {AG Toda},
      ddc          = {570},
      cid          = {I:(DE-2719)1710014},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)7},
      doi          = {10.1007/978-1-0716-4807-0_12},
      url          = {https://pub.dzne.de/record/282545},
}