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@ARTICLE{Oberlnder:282582,
author = {Oberländer, Kristin and Pruunsild, Priit and Koch, Philipp
and Yan, Jing and Szafranski, Karol and Bading, Hilmar},
title = {{I}nhba, {H}omer1 and {B}dnf are major targets of
transcriptomic dysregulation by neurodegenerative
disease-associated excitotoxic {NMDA} receptor signaling.},
journal = {Communications biology},
volume = {8},
number = {1},
issn = {2399-3642},
address = {London},
publisher = {Springer Nature},
reportid = {DZNE-2025-01342},
pages = {1743},
year = {2025},
abstract = {Synaptic activity-regulated gene expression supports
neuroprotection, plasticity, and memory. The transcription
factor CREB is central to these processes. It is activated
by synaptic NMDA receptors but inactivated by excitotoxic
extrasynaptic NMDAR (esNMDAR) signaling. Using primary
hippocampal neurons, we modeled neurodegeneration and found
that esNMDAR activation, which causes CREB shut-off and
inactivation of the ERK/MAPK-ELK1/SRF pathway, extensively
distorted control of synaptic activity over transcription.
This resulted in the suppression of key neuroprotective
genes, in particular Inhba and Bdnf, but also of genes
involved in synaptic function (Homer1, Btg2, Mir132, Mir212)
and transcription factor genes (Atf3, Egr1, Fos, Npas4). In
a Huntington's disease (HD) mouse model, treatment with
memantine or targeting the NMDAR/TRPM4 complex with FP802
restored gene expression, notably Inhba, Homer1 and Bdnf,
and attenuated the decrease of the HD disease marker Ppp1r1b
(DARPP-32). These findings identify esNMDAR-driven
transcriptional dysregulation as a key pathomechanism in
neurodegenerative disease, supporting inhibition of
esNMDAR-signaling as a promising therapeutic approach.},
keywords = {Animals / Brain-Derived Neurotrophic Factor: genetics /
Brain-Derived Neurotrophic Factor: metabolism / Receptors,
N-Methyl-D-Aspartate: metabolism / Receptors,
N-Methyl-D-Aspartate: genetics / Homer Scaffolding Proteins:
genetics / Homer Scaffolding Proteins: metabolism / Mice /
Signal Transduction / Transcriptome / Neurodegenerative
Diseases: metabolism / Neurodegenerative Diseases: genetics
/ Hippocampus: metabolism / Neurons: metabolism / Huntington
Disease: genetics / Huntington Disease: metabolism / Gene
Expression Regulation / Disease Models, Animal / Male /
Mice, Inbred C57BL / Brain-Derived Neurotrophic Factor (NLM
Chemicals) / Receptors, N-Methyl-D-Aspartate (NLM Chemicals)
/ Homer Scaffolding Proteins (NLM Chemicals) / Homer1
protein, mouse (NLM Chemicals) / Bdnf protein, mouse (NLM
Chemicals)},
cin = {AG Schneider},
ddc = {570},
cid = {I:(DE-2719)1011305},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41339520},
doi = {10.1038/s42003-025-09074-9},
url = {https://pub.dzne.de/record/282582},
}