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@ARTICLE{Bader:282589,
      author       = {Bader, Almke and Gao, Jincheng and Reiter, Nina and Siemes,
                      Devon and Zehrer, Annette and Wei, Xiang and Hepper, Ingrid
                      and de Vega Gómez, Enrique and Schmid, Bettina and Forné,
                      Ignasi and Engel, Daniel Robert and Walzog, Barbara and
                      Maier-Begandt, Daniela},
      title        = {{SCAMP}3 is essential for proper formation and function of
                      neutrophil granules.},
      journal      = {Journal of leukocyte biology},
      volume       = {117},
      number       = {12},
      issn         = {0741-5400},
      address      = {Tokyo},
      publisher    = {Oxford University Press},
      reportid     = {DZNE-2025-01349},
      pages        = {qiaf153},
      year         = {2025},
      abstract     = {Host defense functions of neutrophils during infection
                      critically depend on microbicidal and proteolytic proteins
                      stored in primary, secondary, and tertiary granules that are
                      released into the phagosome or into the extracellular space
                      upon degranulation. Granules are generated during
                      granulopoiesis, and impaired granule production or granule
                      protein sorting has been linked to inefficient pathogen
                      clearance resulting in recurrent bacterial and fungal
                      infections. Here, we studied the role of secretory carrier
                      associated membrane protein 3 (SCAMP3) for neutrophil
                      defense functions. We generated Scamp3 knockout (KO) Hoxb8
                      cells and found that killing of Escherichia coli by Scamp3
                      KO Hoxb8 cell-derived neutrophils (dHoxb8 cells) was
                      compromised as compared with control dHoxb8 cells in vitro.
                      Mass spectrometric and Western blot analyses revealed a
                      significant reduction of primary, secondary, and tertiary
                      granule proteins in the genetic absence of Scamp3, resulting
                      in a reduced overall granularity of these cells.
                      Accordingly, degranulation was reduced in Scamp3 KO dHoxb8
                      cells compared with control dHoxb8 cells. Similarly, Scamp3
                      deficiency in zebrafish resulted in reduced neutrophil
                      granularity in comparison with wild-type animals. However,
                      neutrophil migration toward sites of E. coli infection was
                      unaffected in scamp3 KO zebrafish larvae. In summary, SCAMP3
                      represents an important novel player in granule equipment
                      and degranulation, with key functions in neutrophil defense
                      mechanisms during host-pathogen interactions in vitro.},
      keywords     = {Animals / Neutrophils: immunology / Neutrophils: metabolism
                      / Zebrafish / Cell Degranulation: immunology / Membrane
                      Proteins: genetics / Membrane Proteins: immunology /
                      Membrane Proteins: metabolism / Cytoplasmic Granules:
                      metabolism / Cytoplasmic Granules: immunology / Escherichia
                      coli: immunology / Escherichia coli Infections: immunology /
                      Mice / Homeodomain Proteins: genetics / SCAMP3 (Other) /
                      granules (Other) / host defense (Other) / infection (Other)
                      / neutrophils (Other) / Membrane Proteins (NLM Chemicals) /
                      Homeodomain Proteins (NLM Chemicals)},
      cin          = {AG Schmid München},
      ddc          = {570},
      cid          = {I:(DE-2719)1140002},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41187789},
      doi          = {10.1093/jleuko/qiaf153},
      url          = {https://pub.dzne.de/record/282589},
}