000282928 001__ 282928
000282928 005__ 20251218141410.0
000282928 037__ $$aDZNE-2025-01389
000282928 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$udzne
000282928 245__ $$aDataset: Mass-spectrometry analysis of brain endothelial cells (BECs) from Foxf2iECKO and Ctrl mice (Project PXD051839)
000282928 260__ $$bPRoteomics IDEntifications Database$$c2025
000282928 3367_ $$2BibTeX$$aMISC
000282928 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1766063631_31260
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000282928 520__ $$aTo identify the molecular and cellular pathways mediating the effects of Foxf2 in brain endothelial cells (BECs), we performed proteomic analysis of mouse BECs. For this, we applied our previously published BEC enrichment protocol using magnetic-activated cell sorting (MACS) combined with liquid chromatography-mass spectrometry (LC-MS/MS) based proteomics (Todorov-Völgyi et al., 2024) to 6 months old animals. Proteomic analysis of isolated BECs captured a total of 4750 proteins. Out of these, 320 and 434 proteins were significantly up-, and downregulated, respectively in Cdh5-CreERT2;Foxf2fl/fl (Foxf2iECKO) vs Foxf2fl/fl (Ctrl) mice. In GO enrichment analyses of significantly downregulated proteins we found ‘establishment of endothelial barrier’, ‘nitric oxide metabolic process’ and ‘positive regulation of angiogenesis’ to be among the most affected categories. Fold-change ranking of significantly altered proteins marked Tie2 as one of the most strongly downregulated proteins. Notably, several proteins involved in Tie2-regulated processes, including Nos3 and Ptgis (implicated in nitric oxide metabolic process), Rap1b, Tjp1, Cldn5, and Cdh5 (implicated in establishment of endothelial barrier), and Tie2, Eng, and Itgb1 (implicated in angiogenesis) were downregulated in BECs from Foxf2iECKO mice. Collectively, these findings demonstrate a critical role of endothelial Foxf2 in maintaining BBB integrity and Tie2 signaling.
000282928 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000282928 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$udzne
000282928 8564_ $$uhttps://wwwdev.ebi.ac.uk/pride/archive/projects/PXD051838
000282928 909CO $$ooai:pub.dzne.de:282928$$pVDB
000282928 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000282928 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000282928 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000282928 9141_ $$y2025
000282928 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000282928 980__ $$adataset
000282928 980__ $$aVDB
000282928 980__ $$aI:(DE-2719)1110006
000282928 980__ $$aUNRESTRICTED