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@MISC{Mller:282928,
      author       = {Müller, Stephan A and Lichtenthaler, Stefan},
      title        = {{D}ataset: {M}ass-spectrometry analysis of brain
                      endothelial cells ({BEC}s) from {F}oxf2i{ECKO} and {C}trl
                      mice ({P}roject {PXD}051839)},
      publisher    = {PRoteomics IDEntifications Database},
      reportid     = {DZNE-2025-01389},
      year         = {2025},
      abstract     = {To identify the molecular and cellular pathways mediating
                      the effects of Foxf2 in brain endothelial cells (BECs), we
                      performed proteomic analysis of mouse BECs. For this, we
                      applied our previously published BEC enrichment protocol
                      using magnetic-activated cell sorting (MACS) combined with
                      liquid chromatography-mass spectrometry (LC-MS/MS) based
                      proteomics (Todorov-Völgyi et al., 2024) to 6 months old
                      animals. Proteomic analysis of isolated BECs captured a
                      total of 4750 proteins. Out of these, 320 and 434 proteins
                      were significantly up-, and downregulated, respectively in
                      Cdh5-CreERT2;Foxf2fl/fl (Foxf2iECKO) vs Foxf2fl/fl (Ctrl)
                      mice. In GO enrichment analyses of significantly
                      downregulated proteins we found ‘establishment of
                      endothelial barrier’, ‘nitric oxide metabolic process’
                      and ‘positive regulation of angiogenesis’ to be among
                      the most affected categories. Fold-change ranking of
                      significantly altered proteins marked Tie2 as one of the
                      most strongly downregulated proteins. Notably, several
                      proteins involved in Tie2-regulated processes, including
                      Nos3 and Ptgis (implicated in nitric oxide metabolic
                      process), Rap1b, Tjp1, Cldn5, and Cdh5 (implicated in
                      establishment of endothelial barrier), and Tie2, Eng, and
                      Itgb1 (implicated in angiogenesis) were downregulated in
                      BECs from Foxf2iECKO mice. Collectively, these findings
                      demonstrate a critical role of endothelial Foxf2 in
                      maintaining BBB integrity and Tie2 signaling.},
      cin          = {AG Lichtenthaler},
      cid          = {I:(DE-2719)1110006},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)32},
      url          = {https://pub.dzne.de/record/282928},
}