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@MISC{Mller:282930,
author = {Müller, Stephan A and Lichtenthaler, Stefan},
title = {{D}ataset: {A} proteomics resource for human i{PSC}-derived
endothelial cells (i{EC}), smooth muscle cells (i{SMC}),
pericytes (i{PC}) and astrocytes (i{AS}) in comparison to
i{PSC}s and human primary cells - technical replicates
({P}roject {PXD}051959)},
publisher = {PRoteomics IDEntifications Database},
reportid = {DZNE-2025-01391},
year = {2025},
abstract = {Function and integrity of the blood-brain-barrier (BBB) is
crucial for brain homeostasis, and its malfunction
critically contributes to neurovascular and
neurodegenerative disorders, including cerebral small vessel
disease (SVD), a common cause of stroke and vascular
dementia. So far, mechanistic studies on BBB function have
been mostly conducted in mice and non-physiological in vitro
models, which recapitulate disease features, but often lack
complex phenotypes, have limited translatability to humans,
and pose challenges for drug discovery. Therefore, we aimed
to establish a fully iPSC-derived 3D model of the human
blood-brain-barrier. To characterize and validate our
somatic cell differentiation protocols we compared our
iPSC-derived cells with commercially available human primary
cells: brain microvascular endothelial cells (pEC), human
umbilical vein endothelial cells (HUVEC), brain vascular
pericytes (pPC), brain vascular smooth muscle cells (pSMC)
and midbrain astrocytes (pAS).},
cin = {AG Lichtenthaler},
cid = {I:(DE-2719)1110006},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)32},
url = {https://pub.dzne.de/record/282930},
}