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@ARTICLE{deAlmeidaCruz:283017,
      author       = {de Almeida Cruz, Tamires and de Paulo Osorio, Rodrigo and
                      de Menezes Epifanio, Neide Mara and Merghani, Madiha and
                      Pereira, Marcos Dias and de Almeida Chaves, Douglas Siqueira
                      and Outeiro, Tiago Fleming and Riger, Cristiano Jorge},
      title        = {{C}annabis sativa extracts reduce inclusion formation in a
                      cell model of alpha-synuclein aggregation.},
      journal      = {Fitoterapia},
      volume       = {188},
      issn         = {0367-326X},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DZNE-2025-01429},
      pages        = {106968},
      year         = {2025},
      abstract     = {Parkinson's disease (PD) is classified as a synucleinopathy
                      due to the accumulation of protein inclusions rich in the
                      alpha-synuclein (aSyn) protein. Identifying effective
                      pharmacological therapies is important to slow the
                      progression and minimize the symptoms of these diseases.
                      Cannabis sativa has a diverse chemical profile depending on
                      its genotype, including several classes of substances, such
                      as cannabinoids, flavonoids, terpenes, and alkaloids. In
                      this study, we evaluated the effects of four C. sativa
                      extracts with different phytocannabinoid chemical profiles
                      in two cellular models that reproduce alterations in
                      cellular homeostasis common during the cellular phase of PD
                      and other synucleinopathies. We used Saccharomyces
                      cerevisiae strains transformed with plasmid DNA and
                      genetically modified human cells (H4), both expressing aSyn.
                      The results showed that all the extracts were antioxidants,
                      decreasing intracellular oxidation levels and increasing the
                      number of daughter cells in yeast cells, but did not prevent
                      mitochondrial damage. Besides, the extracts reduced the
                      number of intracellular inclusions in H4 cells and increased
                      the number of cells without inclusions. Phytochemical
                      characterization revealed extracts rich in
                      Tetrahydrocannabinol - THC (69.88 $\%),$ Cannabidiol - CBD
                      (52.64 $\%),$ and Cannabinol - CBN (47.38 $\%$ and 58.64
                      $\%),$ and we concluded that, regardless of these
                      percentages, all C. sativa extracts showed protective
                      biological activity against toxicity caused by
                      alpha-synuclein production, both in yeast cells and H4
                      cells.},
      keywords     = {Alpha-synuclein (Other) / Neurodegeneration (Other) /
                      Parkinson's disease, cannabis sativa (Other) / Protein
                      aggregation (Other)},
      cin          = {AG Fischer},
      ddc          = {570},
      cid          = {I:(DE-2719)1410002},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41187864},
      doi          = {10.1016/j.fitote.2025.106968},
      url          = {https://pub.dzne.de/record/283017},
}