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000283031 037__ $$aDZNE-2025-01443
000283031 1001_ $$0P:(DE-2719)9002015$$aChhabra, Akshita$$b0$$udzne
000283031 245__ $$aCondensates of synaptic vesicles and synapsin-1 mediate actin polymerization
000283031 260__ $$bBioStudies$$c2025
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000283031 500__ $$aDeutsche Forschungsgemeinschaft (DFG): MI 2104 SFB 1286/B10 SFB 1286/B02 SFB 1286/B02EC | ERC | HORIZON EUROPE European Research Council (ERC): 101078172HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS): NS078165-12 NS126575-01Deutsche Demenzhilfe: Innovative Minds ProgramGerman Academic Scholarship Foundation: N/A
000283031 520__ $$aNeuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior.
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000283031 7001_ $$0P:(DE-2719)9000582$$aHoffmann, Christian$$b1$$udzne
000283031 7001_ $$0P:(DE-2719)9002012$$aAguilar Perez, Gerard$$b2$$udzne
000283031 7001_ $$0P:(DE-2719)9002676$$aKorobeinikov, Aleksandr$$b3$$udzne
000283031 7001_ $$0P:(DE-HGF)0$$aRentsch, Jakob$$b4
000283031 7001_ $$0P:(DE-HGF)0$$aHümpfer, Nadja$$b5
000283031 7001_ $$0P:(DE-2719)9001437$$aKokwaro, Linda$$b6
000283031 7001_ $$0P:(DE-2719)9002532$$aGnidovec, Luka$$b7$$udzne
000283031 7001_ $$0P:(DE-HGF)0$$aPetrovic, Arsen$$b8
000283031 7001_ $$0P:(DE-HGF)0$$aWallace, Jaqulin$$b9
000283031 7001_ $$0P:(DE-2719)9002092$$aTromm, Johannes Vincent$$b10$$udzne
000283031 7001_ $$0P:(DE-HGF)0$$aRoman-Vendrell, Cristina$$b11
000283031 7001_ $$0P:(DE-HGF)0$$aJohnson, Emma$$b12
000283031 7001_ $$0P:(DE-2719)9001749$$aRankovic, Branislava$$b13$$udzne
000283031 7001_ $$0P:(DE-HGF)0$$aPerego, Eleonora$$b14
000283031 7001_ $$0P:(DE-HGF)0$$aVolpi, Tommaso$$b15
000283031 7001_ $$0P:(DE-HGF)0$$aFernandez-Busnadiego, Ruben$$b16
000283031 7001_ $$0P:(DE-HGF)0$$aKöster, Sarah$$b17
000283031 7001_ $$0P:(DE-HGF)0$$aRizzoli, Silvio$$b18
000283031 7001_ $$0P:(DE-HGF)0$$aEwers, Helge$$b19
000283031 7001_ $$0P:(DE-HGF)0$$aMorgan, Jennifer$$b20
000283031 7001_ $$0P:(DE-2719)9000670$$aMilovanovic, Dragomir$$b21$$eLast author$$udzne
000283031 7870_ $$0DZNE-2025-01105$$aChhabra, Akshita et.al.$$d[London] : Nature Publishing Group UK, 2025$$iRelatedTo$$r$$tCondensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.
000283031 8564_ $$uhttps://www.ebi.ac.uk/biostudies/sourcedata/studies/S-SCDT-10_1038-S44318-025-00516-y
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000283031 9201_ $$0I:(DE-2719)1813002$$kAG Milovanovic (Berlin)$$lMolecular Neuroscience$$x0
000283031 9201_ $$0I:(DE-2719)1013043$$kAG Milovanovic (Bonn)$$lMolecular Neuroscience$$x1
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