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@MISC{Chhabra:283031,
author = {Chhabra, Akshita and Hoffmann, Christian and Aguilar Perez,
Gerard and Korobeinikov, Aleksandr and Rentsch, Jakob and
Hümpfer, Nadja and Kokwaro, Linda and Gnidovec, Luka and
Petrovic, Arsen and Wallace, Jaqulin and Tromm, Johannes
Vincent and Roman-Vendrell, Cristina and Johnson, Emma and
Rankovic, Branislava and Perego, Eleonora and Volpi, Tommaso
and Fernandez-Busnadiego, Ruben and Köster, Sarah and
Rizzoli, Silvio and Ewers, Helge and Morgan, Jennifer and
Milovanovic, Dragomir},
title = {{C}ondensates of synaptic vesicles and synapsin-1 mediate
actin polymerization},
publisher = {BioStudies},
reportid = {DZNE-2025-01443},
year = {2025},
note = {Deutsche Forschungsgemeinschaft (DFG): MI 2104 SFB 1286/B10
SFB 1286/B02 SFB 1286/B02EC | ERC | HORIZON EUROPE European
Research Council (ERC): 101078172HHS | NIH | National
Institute of Neurological Disorders and Stroke (NINDS):
NS078165-12 NS126575-01Deutsche Demenzhilfe: Innovative
Minds ProgramGerman Academic Scholarship Foundation: N/A},
abstract = {Neuronal communication relies on precisely maintained
synaptic vesicle (SV) clusters, which assemble via
liquid-liquid phase separation. This process requires
synapsins, the major synaptic phosphoproteins, which are
known to bind actin. Reorganization of SVs, synapsins, and
actin is a hallmark of synaptic activity, but the molecular
details of the interactions between these components remain
unclear. Here, we combine in vitro reconstitution with
expansion microscopy, super-resolution imaging, and
cryo-electron tomography to dissect the roles of
SV-synapsin-1 condensates in the organization of the
presynaptic actin cytoskeleton. Our results indicate that
condensation of synapsin-1 initiates actin polymerization.
This process enables SV-synapsin-actin assemblies to
facilitate the mesoscale organization of SV clusters along
axons, which is similar to the native presynaptic
organization observed at both lamprey and mammalian
synapses. Understanding the relationship between the actin
network and synapsin-synaptic vesicle condensates can help
elucidate how coordinated neurotransmission along the axon
enables circuit function and behavior.},
cin = {AG Milovanovic (Berlin) / AG Milovanovic (Bonn)},
cid = {I:(DE-2719)1813002 / I:(DE-2719)1013043},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)32},
url = {https://pub.dzne.de/record/283031},
}