TY - JOUR AU - Freri, Fabiola AU - Spinelli, Edoardo Gioele AU - Canu, Elisa AU - Basaia, Silvia AU - Castelnovo, Veronica AU - Müller, Hans-Peter AU - Kassubek, Jan AU - Ludolph, Albert C AU - Krishnamurthy, Sruthi Sankari AU - Roselli, Francesco AU - Filippi, Massimo AU - Agosta, Federica TI - Uncovering hypothalamic network disruption in ALS. JO - Journal of neurology VL - 273 IS - 1 SN - 0367-004X CY - [Darmstadt] PB - Steinkopff M1 - DZNE-2025-01454 SP - 37 PY - 2026 AB - Structural MRI studies have shown hypothalamic atrophy and altered white matter (WM) connectivity in amyotrophic lateral sclerosis (ALS), as a possible substrate of hypermetabolism in this condition. However, hypothalamic functional connectivity and its association with clinical features in ALS remain unclear. This study explored hypothalamic resting-state functional connectivity (RS-FC) in ALS patients compared to controls and its relationship with disease severity defined by the ALS Functional Rating Scale (ALSFRS-r), body mass index (BMI), disease duration, progression rate, survival, hypothalamic volume, and WM integrity.Seventy-one ALS patients and 39 healthy controls underwent structural and RS functional MRI. The bilateral hypothalamus was segmented, and a seed-based RS-FC analysis was performed. Group differences in hypothalamic RS-FC and their correlations with ALSFRS-r scores, BMI, disease duration, progression rate, survival, hypothalamic volume, and WM integrity were assessed. Tract-based spatial statistics was performed to estimate the correlation between WM damage in ALS and hypothalamic RS-FC.ALS patients showed increased hypothalamic RS-FC with caudate nuclei compared to controls. Additionally, greater disease severity correlated with increased hypothalamic RS-FC with the caudate nuclei and orbitofrontal cortex. Hypothalamic RS-FC mean values also associated with FA in the genu of corpus callosum and forceps minor and disease progression rate. No significant correlations were observed with other clinical features.These findings support hypothalamic alterations in ALS. Early detection of hypothalamic changes could be useful in prognostic stratification and evaluating intervention effects. KW - Humans KW - Amyotrophic Lateral Sclerosis: diagnostic imaging KW - Amyotrophic Lateral Sclerosis: physiopathology KW - Amyotrophic Lateral Sclerosis: pathology KW - Female KW - Male KW - Middle Aged KW - Hypothalamus: diagnostic imaging KW - Hypothalamus: physiopathology KW - Hypothalamus: pathology KW - Magnetic Resonance Imaging KW - Aged KW - Adult KW - White Matter: diagnostic imaging KW - White Matter: pathology KW - Nerve Net: diagnostic imaging KW - Nerve Net: physiopathology KW - Disease Progression KW - Connectome KW - Amyotrophic lateral sclerosis (Other) KW - Functional connectivity (Other) KW - Hypermetabolism (Other) KW - Hypothalamus (Other) KW - MRI (Other) LB - PUB:(DE-HGF)16 C6 - pmid:41428120 C2 - pmc:PMC12722382 DO - DOI:10.1007/s00415-025-13574-3 UR - https://pub.dzne.de/record/283047 ER -