000283048 001__ 283048
000283048 005__ 20260107144733.0
000283048 0247_ $$2doi$$a10.1073/pnas.2513375122
000283048 0247_ $$2pmid$$apmid:41428877
000283048 0247_ $$2ISSN$$a0027-8424
000283048 0247_ $$2ISSN$$a1091-6490
000283048 037__ $$aDZNE-2025-01455
000283048 041__ $$aEnglish
000283048 082__ $$a500
000283048 1001_ $$00000-0001-7368-5539$$aVierock, Johannes$$b0
000283048 245__ $$aMulticolor photoreactions of the red light-activated channelrhodopsin Chrimson.
000283048 260__ $$aWashington, DC$$bNational Acad. of Sciences$$c2025
000283048 3367_ $$2DRIVER$$aarticle
000283048 3367_ $$2DataCite$$aOutput Types/Journal article
000283048 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1767004423_31203
000283048 3367_ $$2BibTeX$$aARTICLE
000283048 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000283048 3367_ $$00$$2EndNote$$aJournal Article
000283048 520__ $$aChannelrhodopsins are light-gated ion channels that are used in modern neurosciences for the precise control of cellular ion fluxes by light. With a peak absorption at 585 nm, Chrimson is the most red-shifted cation-conducting ChR. It is frequently employed in multicolor experiments alongside blue light-sensitive optogenetic tools and is so far the only light-gated ion channel successfully applied in human vision restoration. However, its photoresponses to different wavelengths have not been thoroughly characterized. In this study, we identify multiple interconvertible dark states of Chrimson with distinct absorption and photokinetic properties. Combining electrophysiology and spectroscopy with optogenetic experiments in neurons, we unveil that this dark state heterogeneity is based on distinct protonation dynamics of the counterion complex and alternative retinal isomerization. In neurons, prolonged red illumination reduces Chrimson's red light sensitivity, which is reflected by a blue shift of the action spectrum. Blue light pulses reverse this shift and increase the excitability in subsequent red-light flashes. This understanding of wavelength-dependent photoreactions in Chrimson will improve the design of multicolor optogenetic experiments and inform strategies for optimizing Chrimson for therapeutic applications.
000283048 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000283048 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000283048 650_7 $$2Other$$aChrimson
000283048 650_7 $$2Other$$aFTIR spectroscopy
000283048 650_7 $$2Other$$achannelrhodopsins
000283048 650_7 $$2Other$$aoptogenetics
000283048 650_7 $$2Other$$aretinal prosthesis
000283048 650_7 $$2NLM Chemicals$$aChannelrhodopsins
000283048 650_2 $$2MeSH$$aChannelrhodopsins: metabolism
000283048 650_2 $$2MeSH$$aChannelrhodopsins: chemistry
000283048 650_2 $$2MeSH$$aChannelrhodopsins: genetics
000283048 650_2 $$2MeSH$$aLight
000283048 650_2 $$2MeSH$$aOptogenetics: methods
000283048 650_2 $$2MeSH$$aNeurons: metabolism
000283048 650_2 $$2MeSH$$aNeurons: radiation effects
000283048 650_2 $$2MeSH$$aHumans
000283048 650_2 $$2MeSH$$aAnimals
000283048 650_2 $$2MeSH$$aHEK293 Cells
000283048 650_2 $$2MeSH$$aRed Light
000283048 7001_ $$aKaufmann, Joel C D$$b1
000283048 7001_ $$0P:(DE-2719)9001061$$aFaiß, Lukas$$b2$$eFirst author$$udzne
000283048 7001_ $$aTillert, Linda$$b3
000283048 7001_ $$aKrause, Benjamin S$$b4
000283048 7001_ $$aFischer, Paul$$b5
000283048 7001_ $$aNguyen, Thi Bich Thao$$b6
000283048 7001_ $$0P:(DE-2719)2810725$$aSchmitz, Dietmar$$b7
000283048 7001_ $$0P:(DE-2719)2810914$$aRost, Benjamin R$$b8$$udzne
000283048 7001_ $$aBartl, Franz$$b9
000283048 7001_ $$00000-0003-3589-6452$$aHegemann, Peter$$b10
000283048 773__ $$0PERI:(DE-600)1461794-8$$a10.1073/pnas.2513375122$$gVol. 122, no. 52, p. e2513375122$$n52$$pe2513375122$$tProceedings of the National Academy of Sciences of the United States of America$$v122$$x0027-8424$$y2025
000283048 8564_ $$uhttps://pub.dzne.de/record/283048/files/DZNE-2025-1455.pdf$$yOpenAccess
000283048 8564_ $$uhttps://pub.dzne.de/record/283048/files/DZNE-2025-1455.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000283048 909CO $$ooai:pub.dzne.de:283048$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000283048 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001061$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000283048 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810725$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b7$$kDZNE
000283048 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810914$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b8$$kDZNE
000283048 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000283048 9141_ $$y2025
000283048 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bP NATL ACAD SCI USA : 2022$$d2024-12-10
000283048 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000283048 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bP NATL ACAD SCI USA : 2022$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000283048 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0430$$2StatID$$aNational-Konsortium$$d2024-12-10$$wger
000283048 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-10
000283048 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-10
000283048 9201_ $$0I:(DE-2719)1810004$$kAG Schmitz$$lNetwork Dysfunction$$x0
000283048 980__ $$ajournal
000283048 980__ $$aVDB
000283048 980__ $$aUNRESTRICTED
000283048 980__ $$aI:(DE-2719)1810004
000283048 9801_ $$aFullTexts