Home > Publications Database > Chimeric human organoid and mouse brain slice co-cultures to study microglial function. > print

001     283103
005     20260217130050.0
024 7 _ |2 doi
|a 10.1016/j.celrep.2025.116656
024 7 _ |2 pmid
|a pmid:41385370
024 7 _ |2 ISSN
|a 2211-1247
024 7 _ |2 ISSN
|a 2639-1856
037 _ _ |a DZNE-2025-01510
041 _ _ |a English
082 _ _ |a 610
100 1 _ |0 P:(DE-2719)2813672
|a Panagiotakopoulou, Vasiliki
|b 0
|e First author
|u dzne
245 _ _ |a Chimeric human organoid and mouse brain slice co-cultures to study microglial function.
260 _ _ |a Maryland Heights, MO
|b Cell Press
|c 2025
336 7 _ |2 DRIVER
|a article
336 7 _ |2 DataCite
|a Output Types/Journal article
336 7 _ |0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
|a Journal Article
|b journal
|m journal
|s 1767103605_6286
336 7 _ |2 BibTeX
|a ARTICLE
336 7 _ |2 ORCID
|a JOURNAL_ARTICLE
336 7 _ |0 0
|2 EndNote
|a Journal Article
520 _ _ |a Studying the dynamic role of microglia in brain development and neurodegenerative diseases requires models that closely resemble the human brain environment. While human induced pluripotent stem cell (iPSC)-derived organoids (hORGs) effectively reproduce key neuronal and certain glial cell types, modeling human microglia in vitro remains challenging. Inspired by recent approaches demonstrating enhanced microglial maturation in hORGs transplanted into mouse brains, we develop a chimeric model by co-culturing hORGs with mouse brain slice cultures (mBSCs). This system reveals cross-species interactions associated with an earlier onset of cortical neuronal differentiation markers in the hORGs. Human iPSC-derived microglia, pre-differentiated in mBSCs, migrate into the hORGs and adopt ramified morphology. They remain viable for several months and respond to laser-induced injury, demonstrating long-term functionality. This in vitro model supports long-term study of human microglia in a brain-like environment, providing a platform for mechanistic studies and screening compounds that target microglial function.
536 _ _ |0 G:(DE-HGF)POF4-351
|a 351 - Brain Function (POF4-351)
|c POF4-351
|f POF IV
|x 0
536 _ _ |0 G:(DE-HGF)POF4-352
|a 352 - Disease Mechanisms (POF4-352)
|c POF4-352
|f POF IV
|x 1
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |2 Other
|a CP: neuroscience
650 _ 7 |2 Other
|a CP: stem cell research
650 _ 7 |2 Other
|a cerebral organoids
650 _ 7 |2 Other
|a chimeric in vitro model
650 _ 7 |2 Other
|a human microglia
650 _ 7 |2 Other
|a xenotransplantation
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Microglia: cytology
650 _ 2 |2 MeSH
|a Microglia: metabolism
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Organoids: cytology
650 _ 2 |2 MeSH
|a Organoids: metabolism
650 _ 2 |2 MeSH
|a Brain: cytology
650 _ 2 |2 MeSH
|a Brain: metabolism
650 _ 2 |2 MeSH
|a Coculture Techniques: methods
650 _ 2 |2 MeSH
|a Induced Pluripotent Stem Cells: cytology
650 _ 2 |2 MeSH
|a Induced Pluripotent Stem Cells: metabolism
650 _ 2 |2 MeSH
|a Mice
650 _ 2 |2 MeSH
|a Cell Differentiation
650 _ 2 |2 MeSH
|a Neurons: cytology
650 _ 2 |2 MeSH
|a Neurons: metabolism
700 1 _ |0 P:(DE-2719)9000948
|a Welzer, Marc
|b 1
700 1 _ |0 P:(DE-HGF)0
|a Ormaechea, Olmo Ruiz
|b 2
700 1 _ |0 P:(DE-2719)9002990
|a Werner, Diana
|b 3
|u dzne
700 1 _ |0 P:(DE-2719)9000542
|a Erlebach, Lena
|b 4
|u dzne
700 1 _ |0 P:(DE-2719)2813030
|a Bühler, Anika
|b 5
|u dzne
700 1 _ |0 P:(DE-2719)2814201
|a Obermüller, Ulrike
|b 6
700 1 _ |0 P:(DE-2719)2811021
|a Neher, Jonas J
|b 7
|u dzne
700 1 _ |0 P:(DE-2719)2000010
|a Jucker, Mathias
|b 8
|u dzne
700 1 _ |0 P:(DE-2719)9001451
|a Kronenberg-Versteeg, Deborah
|b 9
|e Last author
|u dzne
773 _ _ |0 PERI:(DE-600)2649101-1
|a 10.1016/j.celrep.2025.116656
|g Vol. 44, no. 12, p. 116656 -
|n 12
|p 116656
|t Cell reports
|v 44
|x 2211-1247
|y 2025
856 4 _ |u https://pub.dzne.de/record/283103/files/DZNE-2025-1510.pdf
|y OpenAccess
856 4 _ |u https://pub.dzne.de/record/283103/files/DZNE-2025-1510.pdf?subformat=pdfa
|x pdfa
|y OpenAccess
909 C O |o oai:pub.dzne.de:283103
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2813672
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 0
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)9000948
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 1
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-HGF)0
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 2
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)9002990
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 3
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)9000542
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 4
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2813030
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 5
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2814201
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 6
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2811021
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 7
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2000010
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 8
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)9001451
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 9
|k DZNE
913 1 _ |0 G:(DE-HGF)POF4-351
|1 G:(DE-HGF)POF4-350
|2 G:(DE-HGF)POF4-300
|3 G:(DE-HGF)POF4
|4 G:(DE-HGF)POF
|a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|v Brain Function
|x 0
913 1 _ |0 G:(DE-HGF)POF4-352
|1 G:(DE-HGF)POF4-350
|2 G:(DE-HGF)POF4-300
|3 G:(DE-HGF)POF4
|4 G:(DE-HGF)POF
|a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|v Disease Mechanisms
|x 1
914 1 _ |y 2025
915 _ _ |0 StatID:(DE-HGF)0200
|2 StatID
|a DBCoverage
|b SCOPUS
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0160
|2 StatID
|a DBCoverage
|b Essential Science Indicators
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)1050
|2 StatID
|a DBCoverage
|b BIOSIS Previews
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)1190
|2 StatID
|a DBCoverage
|b Biological Abstracts
|d 2024-12-16
915 _ _ |0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
|a Creative Commons Attribution CC BY 4.0
915 _ _ |0 StatID:(DE-HGF)0100
|2 StatID
|a JCR
|b CELL REP : 2022
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0501
|2 StatID
|a DBCoverage
|b DOAJ Seal
|d 2023-05-02T08:49:39Z
915 _ _ |0 StatID:(DE-HGF)0500
|2 StatID
|a DBCoverage
|b DOAJ
|d 2023-05-02T08:49:39Z
915 _ _ |0 StatID:(DE-HGF)0113
|2 StatID
|a WoS
|b Science Citation Index Expanded
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0700
|2 StatID
|a Fees
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0150
|2 StatID
|a DBCoverage
|b Web of Science Core Collection
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0510
|2 StatID
|a OpenAccess
915 _ _ |0 StatID:(DE-HGF)0030
|2 StatID
|a Peer Review
|b DOAJ : Anonymous peer review
|d 2023-05-02T08:49:39Z
915 _ _ |0 StatID:(DE-HGF)0561
|2 StatID
|a Article Processing Charges
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)9905
|2 StatID
|a IF >= 5
|b CELL REP : 2022
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0300
|2 StatID
|a DBCoverage
|b Medline
|d 2024-12-16
915 _ _ |0 StatID:(DE-HGF)0199
|2 StatID
|a DBCoverage
|b Clarivate Analytics Master Journal List
|d 2024-12-16
920 1 _ |0 I:(DE-2719)1210015
|k AG Kronenberg-Versteeg
|l Glial Cell Biology
|x 0
920 1 _ |0 I:(DE-2719)1210001
|k AG Jucker
|l Cell Biology of Neurological Diseases
|x 1
920 1 _ |0 I:(DE-2719)1210012
|k AG Neher (Tübingen)
|l Neuroimmunology and Neurodegenerative Disease
|x 2
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-2719)1210015
980 _ _ |a I:(DE-2719)1210001
980 _ _ |a I:(DE-2719)1210012
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21