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000283121 020__ $$a978-1-0716-5018-9 (print)
000283121 020__ $$a978-1-0716-5019-6 (electronic)
000283121 0247_ $$2doi$$a10.1007/978-1-0716-5019-6_9
000283121 0247_ $$2pmid$$apmid:41478936
000283121 0247_ $$2ISSN$$a1064-3745
000283121 0247_ $$2ISSN$$a1940-6029
000283121 037__ $$aDZNE-2026-00017
000283121 041__ $$aEnglish
000283121 082__ $$a570
000283121 1001_ $$0P:(DE-2719)9001782$$aSecchim Ribeiro, Lucas$$b0$$eFirst author
000283121 245__ $$aBridging Immunity and Hemostasis: Technical Foundations of Platelet-Leukocyte Co-cultures.
000283121 260__ $$aNew York, NY$$bSpringer US$$c2026
000283121 29510 $$aModels in Inflammation and Immunopharmacology / Secchim Ribeiro, Lucas (Editor) ; New York, NY : Springer US, 2026, Chapter 9 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-5018-9=978-1-0716-5019-6 ; doi:10.1007/978-1-0716-5019-6
000283121 300__ $$a111 - 126
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000283121 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1767364687_32514
000283121 4900_ $$aMethods in Molecular Biology$$v2993
000283121 520__ $$aPlatelets are immune cells with a great potential for the discovery of novel mechanisms regulating both innate and adaptive responses. Although it is known that the interaction between platelets and leukocytes plays a critical role in inflammation and thrombosis, the vast majority of experimental models focus on monotypic cell systems, ignoring the constant association of these two cell types in circulation and in the tissues. Co-culture systems that recapitulate this interplay are essential for understanding cellular processes and for evaluating therapeutic interventions. However, the technical requirements for isolating and handling these cells with such distinct physical and functional properties pose significant challenges. Here I provide a detailed guide for the isolation, characterization, and co-culture of human and murine platelets with leukocytes, while describing systematic procedures for obtaining high-purity platelet suspensions with minimal activation. Parallel instructions for isolating and differentiating leukocytes from human blood or mouse bone marrow are also provided. In addition, I outline flow cytometry-based methods to assess platelet purity and activation status using constitutive and activation-inducible markers. This comprehensive guide is aimed at experienced operators and newcomers alike, offering practical tips and troubleshooting advice to ensure reproducibility and biological relevance. By standardizing critical steps in co-culture protocols, this resource seeks to support high-quality experimental design in immunology and hemostasis research.
000283121 536__ $$0G:(DE-HGF)POF4-354$$a354 - Disease Prevention and Healthy Aging (POF4-354)$$cPOF4-354$$fPOF IV$$x0
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000283121 650_7 $$2Other$$aCo-culture
000283121 650_7 $$2Other$$aImmune response
000283121 650_7 $$2Other$$aInflammation
000283121 650_7 $$2Other$$aLeukocytes
000283121 650_7 $$2Other$$aPlatelets
000283121 650_2 $$2MeSH$$aBlood Platelets: cytology
000283121 650_2 $$2MeSH$$aBlood Platelets: immunology
000283121 650_2 $$2MeSH$$aBlood Platelets: metabolism
000283121 650_2 $$2MeSH$$aCoculture Techniques: methods
000283121 650_2 $$2MeSH$$aHumans
000283121 650_2 $$2MeSH$$aAnimals
000283121 650_2 $$2MeSH$$aMice
000283121 650_2 $$2MeSH$$aLeukocytes: cytology
000283121 650_2 $$2MeSH$$aLeukocytes: immunology
000283121 650_2 $$2MeSH$$aLeukocytes: metabolism
000283121 650_2 $$2MeSH$$aHemostasis
000283121 650_2 $$2MeSH$$aFlow Cytometry: methods
000283121 650_2 $$2MeSH$$aCell Separation: methods
000283121 650_2 $$2MeSH$$aPlatelet Activation
000283121 773__ $$a10.1007/978-1-0716-5019-6_9
000283121 8564_ $$uhttps://pub.dzne.de/record/283121/files/DZNE-2026-00017_Restricted.pdf
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000283121 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001782$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
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000283121 9141_ $$y2026
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000283121 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-19
000283121 9201_ $$0I:(DE-2719)1013038$$kAG Schultze$$lClinical Single Cell Omics (CSCO) / Systems Medicine$$x0
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