% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INBOOK{SecchimRibeiro:283121,
      author       = {Secchim Ribeiro, Lucas},
      title        = {{B}ridging {I}mmunity and {H}emostasis: {T}echnical
                      {F}oundations of {P}latelet-{L}eukocyte {C}o-cultures.},
      volume       = {2993},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {DZNE-2026-00017},
      isbn         = {978-1-0716-5018-9 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {111 - 126},
      year         = {2026},
      comment      = {Models in Inflammation and Immunopharmacology / Secchim
                      Ribeiro, Lucas (Editor) ; New York, NY : Springer US, 2026,
                      Chapter 9 ; ISSN: 1064-3745=1940-6029 ; ISBN:
                      978-1-0716-5018-9=978-1-0716-5019-6 ;
                      doi:10.1007/978-1-0716-5019-6},
      booktitle     = {Models in Inflammation and
                       Immunopharmacology / Secchim Ribeiro,
                       Lucas (Editor) ; New York, NY :
                       Springer US, 2026, Chapter 9 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-5018-9=978-1-0716-5019-6 ;
                       doi:10.1007/978-1-0716-5019-6},
      abstract     = {Platelets are immune cells with a great potential for the
                      discovery of novel mechanisms regulating both innate and
                      adaptive responses. Although it is known that the
                      interaction between platelets and leukocytes plays a
                      critical role in inflammation and thrombosis, the vast
                      majority of experimental models focus on monotypic cell
                      systems, ignoring the constant association of these two cell
                      types in circulation and in the tissues. Co-culture systems
                      that recapitulate this interplay are essential for
                      understanding cellular processes and for evaluating
                      therapeutic interventions. However, the technical
                      requirements for isolating and handling these cells with
                      such distinct physical and functional properties pose
                      significant challenges. Here I provide a detailed guide for
                      the isolation, characterization, and co-culture of human and
                      murine platelets with leukocytes, while describing
                      systematic procedures for obtaining high-purity platelet
                      suspensions with minimal activation. Parallel instructions
                      for isolating and differentiating leukocytes from human
                      blood or mouse bone marrow are also provided. In addition, I
                      outline flow cytometry-based methods to assess platelet
                      purity and activation status using constitutive and
                      activation-inducible markers. This comprehensive guide is
                      aimed at experienced operators and newcomers alike, offering
                      practical tips and troubleshooting advice to ensure
                      reproducibility and biological relevance. By standardizing
                      critical steps in co-culture protocols, this resource seeks
                      to support high-quality experimental design in immunology
                      and hemostasis research.},
      keywords     = {Blood Platelets: cytology / Blood Platelets: immunology /
                      Blood Platelets: metabolism / Coculture Techniques: methods
                      / Humans / Animals / Mice / Leukocytes: cytology /
                      Leukocytes: immunology / Leukocytes: metabolism / Hemostasis
                      / Flow Cytometry: methods / Cell Separation: methods /
                      Platelet Activation / Co-culture (Other) / Immune response
                      (Other) / Inflammation (Other) / Leukocytes (Other) /
                      Platelets (Other)},
      cin          = {AG Schultze},
      ddc          = {570},
      cid          = {I:(DE-2719)1013038},
      pnm          = {354 - Disease Prevention and Healthy Aging (POF4-354)},
      pid          = {G:(DE-HGF)POF4-354},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:41478936},
      doi          = {10.1007/978-1-0716-5019-6_9},
      url          = {https://pub.dzne.de/record/283121},
}