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@ARTICLE{Tsai:284365,
author = {Tsai, Wei-Yun and Giesbertz, Pieter and Breimann, Stephan
and Lichtenthaler, Stefan and Frishman, Dmitrij},
title = {{M}ulti-platform integration of brain and {CSF} proteomes
reveals biomarker panels for {A}lzheimer's disease.},
journal = {Briefings in bioinformatics},
volume = {27},
number = {1},
issn = {1467-5463},
address = {Oxford [u.a.]},
publisher = {Oxford University Press},
reportid = {DZNE-2026-00133},
pages = {bbag012},
year = {2026},
abstract = {Alzheimer's disease (AD) is the leading cause of dementia
and represents a progressive, irreversible neurodegenerative
disorder. Given the complexity and heterogeneity of AD,
which involves numerous interrelated molecular pathways,
large-scale proteomics datasets are essential for robust
biomarker discovery. Comprehensive proteomic profiling
enables the unbiased identification of novel biomarkers
across diverse biological processes, thereby increasing the
likelihood of finding sensitive and specific candidates for
early diagnosis and therapeutic targeting. In this study, we
analyzed 28 large-scale proteomics datasets obtained from
the AD Knowledge Portal and published studies. The data
comprise tandem mass tag, label-free quantification, and
proximity extension assay measurements from brain tissue and
cerebrospinal fluid. To enhance analytical power, we
integrated these proteomic profiles with corresponding
clinical information to construct comprehensive feature sets
for subsequent machine learning analysis. Using Random
Forest and Logistic Regression models, we identified a panel
of proteins capable of distinguishing AD patients from
healthy controls. Several of these biomarkers have been
previously validated in the context of AD, while others
represent novel candidates not yet reported as
AD-associated. These newly identified biomarkers warrant
further experimental validation and hold promise for
improving early diagnosis as well as guiding the development
of targeted therapies for AD.},
keywords = {Alzheimer Disease: metabolism / Alzheimer Disease:
cerebrospinal fluid / Alzheimer Disease: diagnosis / Humans
/ Biomarkers: cerebrospinal fluid / Biomarkers: metabolism /
Proteome: metabolism / Brain: metabolism / Proteomics:
methods / Machine Learning / CSF (Other) / biomarkers
(Other) / brain tissue (Other) / data integration (Other) /
machine learning (Other) / proteomics (Other) / Biomarkers
(NLM Chemicals) / Proteome (NLM Chemicals)},
cin = {AG Lichtenthaler / AG Steiner},
ddc = {004},
cid = {I:(DE-2719)1110006 / I:(DE-2719)1110000-1},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41608987},
doi = {10.1093/bib/bbag012},
url = {https://pub.dzne.de/record/284365},
}