TY  - JOUR
AU  - Daugherty, Ana M
AU  - Carr, Valerie
AU  - Canada, Kelsey L
AU  - Rådman, Gustaf
AU  - Brown, Thackery
AU  - Augustinack, Jean
AU  - Amunts, Katrin
AU  - Bakker, Arnold
AU  - Berron, David
AU  - Burggren, Alison
AU  - Chetelat, Gael
AU  - de Flores, Robin
AU  - Ding, Song-Lin
AU  - Huang, Yushan
AU  - Johnson, Elliott
AU  - Kanel, Prabesh
AU  - Keresztes, Attila
AU  - Kedo, Olga
AU  - Kennedy, Kristen M
AU  - Lee, Joshua
AU  - Malykhin, Nikolai
AU  - Martinez, Anjelica
AU  - Mueller, Susanne
AU  - Mulligan, Elizabeth
AU  - Ofen, Noa
AU  - Palombo, Daniela
AU  - Pasquini, Lorenzo
AU  - Pluta, John
AU  - Raz, Naftali
AU  - Riggins, Tracy
AU  - Rodrigue, Karen M
AU  - Saifullah, Samaah
AU  - Schlichting, Margaret L
AU  - Stark, Craig
AU  - Wang, Lei
AU  - Yushkevich, Paul
AU  - La Joie, Renaud
AU  - Wisse, Laura
AU  - Olsen, Rosanna
TI  - Harmonized Protocol for Subfield Segmentation in the Hippocampal Body on High-Resolution In Vivo MRI From the Hippocampal Subfields Group (HSG).
JO  - Hippocampus
VL  - 36
IS  - 2
SN  - 1050-9631
CY  - New York, NY [u.a.]
PB  - Wiley
M1  - DZNE-2026-00149
SP  - e70073
PY  - 2026
AB  - Hippocampal subfields differentially develop and age, and they vary in vulnerability to neurodegenerative diseases. Innovation in high-resolution imaging has accelerated clinical research on human hippocampal subfields, but substantial differences in segmentation protocols impede comparisons of results across laboratories. The Hippocampal Subfields Group (HSG) is an international organization seeking to address this issue by developing a histologically valid, reliable, and freely available segmentation protocol for high-resolution T2-weighted 3 T MRI (http://www.hippocampalsubfields.com). Here, we report the first portion of the protocol focused on subfields in the hippocampal body; protocols for the head and tail are in development. The body protocol includes definitions of the internal boundaries between subiculum, Cornu Ammonis (CA) 1-3 subfields, and dentate gyrus, in addition to the external boundaries of the hippocampus apart from surrounding white matter and cerebrospinal fluid. The segmentation protocol is based on a novel histological reference dataset labeled by multiple expert neuroanatomists. With broad participation of the research community, we voted on the segmentation protocol via an online survey, which included detailed protocol information, feasibility testing, demonstration videos, example segmentations, and labeled histology. All boundary definitions were rated as having high clarity and reached consensus agreement by Delphi procedure. The harmonized body protocol yielded high inter- and intra-rater reliability. In the present paper we report the procedures to develop and test the protocol, as well as the detailed procedures for manual segmentation using the harmonized protocol. The harmonized protocol will significantly facilitate cross-study comparisons and provide increased insight into the structure and function of hippocampal subfields across the lifespan and in neurodegenerative diseases.
KW  - Hippocampus: diagnostic imaging
KW  - Hippocampus: anatomy & histology
KW  - Magnetic Resonance Imaging: methods
KW  - Magnetic Resonance Imaging: standards
KW  - Humans
KW  - Image Processing, Computer-Assisted: methods
KW  - Male
KW  - Female
KW  - Cornu Ammonis (Other)
KW  - dentate gyrus (Other)
KW  - neuroimaging (Other)
KW  - subiculum (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:41635209
DO  - DOI:10.1002/hipo.70073
UR  - https://pub.dzne.de/record/285024
ER  -