%0 Book Section
%A Hartmann, Celia L
%A Schultze, Joachim L
%A Beyer, Marc D
%A Carraro, Caterina
%T Laser-Capture Microdissection Followed by ATAC-Seq.
%V 3015
%C New York, NY
%I Springer US
%M DZNE-2026-00151
%@ 978-1-0716-5153-7 (print)
%B Methods in Molecular Biology
%P 151 - 162
%D 2026
%< Laser Capture Microdissection / Noberini, Roberta (Editor) ; New York, NY : Springer US, 2026, Chapter 12 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-5153-7=978-1-0716-5154-4 ; doi:10.1007/978-1-0716-5154-4
%X Laser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples.
%K Laser Capture Microdissection: methods
%K Chromatin: genetics
%K Chromatin Immunoprecipitation Sequencing: methods
%K Humans
%K High-Throughput Nucleotide Sequencing: methods
%K ATAC-seq (Other)
%K Chromatin accessibility (Other)
%K Laser-capture microdissection (Other)
%K Morphological staining (Other)
%K Spatial omics (Other)
%K Chromatin (NLM Chemicals)
%F PUB:(DE-HGF)7
%9 Contribution to a book
%$ pmid:41629717
%R 10.1007/978-1-0716-5154-4_12
%U https://pub.dzne.de/record/285026