000285026 001__ 285026 000285026 005__ 20260205153658.0 000285026 020__ $$a978-1-0716-5153-7 (print) 000285026 020__ $$a978-1-0716-5154-4 (electronic) 000285026 0247_ $$2doi$$a10.1007/978-1-0716-5154-4_12 000285026 0247_ $$2pmid$$apmid:41629717 000285026 0247_ $$2ISSN$$a1064-3745 000285026 0247_ $$2ISSN$$a1940-6029 000285026 037__ $$aDZNE-2026-00151 000285026 041__ $$aEnglish 000285026 082__ $$a570 000285026 1001_ $$0P:(DE-2719)9002121$$aHartmann, Celia L$$b0$$eFirst author$$udzne 000285026 245__ $$aLaser-Capture Microdissection Followed by ATAC-Seq. 000285026 260__ $$aNew York, NY$$bSpringer US$$c2026 000285026 29510 $$aLaser Capture Microdissection / Noberini, Roberta (Editor) ; New York, NY : Springer US, 2026, Chapter 12 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-5153-7=978-1-0716-5154-4 ; doi:10.1007/978-1-0716-5154-4 000285026 300__ $$a151 - 162 000285026 3367_ $$2ORCID$$aBOOK_CHAPTER 000285026 3367_ $$07$$2EndNote$$aBook Section 000285026 3367_ $$2DRIVER$$abookPart 000285026 3367_ $$2BibTeX$$aINBOOK 000285026 3367_ $$2DataCite$$aOutput Types/Book chapter 000285026 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1770302013_11292 000285026 4900_ $$aMethods in Molecular Biology$$v3015 000285026 520__ $$aLaser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples. 000285026 536__ $$0G:(DE-HGF)POF4-354$$a354 - Disease Prevention and Healthy Aging (POF4-354)$$cPOF4-354$$fPOF IV$$x0 000285026 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x1 000285026 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x2 000285026 588__ $$aDataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de 000285026 650_7 $$2Other$$aATAC-seq 000285026 650_7 $$2Other$$aChromatin accessibility 000285026 650_7 $$2Other$$aLaser-capture microdissection 000285026 650_7 $$2Other$$aMorphological staining 000285026 650_7 $$2Other$$aSpatial omics 000285026 650_7 $$2NLM Chemicals$$aChromatin 000285026 650_2 $$2MeSH$$aLaser Capture Microdissection: methods 000285026 650_2 $$2MeSH$$aChromatin: genetics 000285026 650_2 $$2MeSH$$aChromatin Immunoprecipitation Sequencing: methods 000285026 650_2 $$2MeSH$$aHumans 000285026 650_2 $$2MeSH$$aHigh-Throughput Nucleotide Sequencing: methods 000285026 693__ $$0EXP:(DE-2719)PRECISE-20190321$$5EXP:(DE-2719)PRECISE-20190321$$ePlatform for Single Cell Genomics and Epigenomics at DZNE University of Bonn$$x0 000285026 7001_ $$0P:(DE-2719)2811660$$aSchultze, Joachim L$$b1$$udzne 000285026 7001_ $$0P:(DE-2719)2812219$$aBeyer, Marc D$$b2$$udzne 000285026 7001_ $$0P:(DE-2719)9001303$$aCarraro, Caterina$$b3$$eLast author$$udzne 000285026 773__ $$a10.1007/978-1-0716-5154-4_12 000285026 8564_ $$uhttps://pub.dzne.de/record/285026/files/DZNE-2026-00151_Restricted.pdf 000285026 8564_ $$uhttps://pub.dzne.de/record/285026/files/DZNE-2026-00151_Restricted.pdf?subformat=pdfa$$xpdfa 000285026 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9002121$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE 000285026 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811660$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE 000285026 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812219$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE 000285026 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001303$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE 000285026 9131_ $$0G:(DE-HGF)POF4-354$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Prevention and Healthy Aging$$x0 000285026 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x1 000285026 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x2 000285026 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-11-05 000285026 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-11-05 000285026 9201_ $$0I:(DE-2719)1013038$$kAG Schultze$$lClinical Single Cell Omics (CSCO) / Systems Medicine$$x0 000285026 9201_ $$0I:(DE-2719)1013035$$kAG Beyer$$lImmunogenomics and Neurodegeneration$$x1 000285026 9201_ $$0I:(DE-2719)1013031$$kPRECISE$$lPlatform for Single Cell Genomics and Epigenomics$$x2 000285026 980__ $$acontb 000285026 980__ $$aEDITORS 000285026 980__ $$aVDBINPRINT 000285026 980__ $$aI:(DE-2719)1013038 000285026 980__ $$aI:(DE-2719)1013035 000285026 980__ $$aI:(DE-2719)1013031 000285026 980__ $$aUNRESTRICTED