TY  - CHAP
AU  - Hartmann, Celia L
AU  - Schultze, Joachim L
AU  - Beyer, Marc D
AU  - Carraro, Caterina
TI  - Laser-Capture Microdissection Followed by ATAC-Seq.
VL  - 3015
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2026-00151
SN  - 978-1-0716-5153-7 (print)
T2  - Methods in Molecular Biology
SP  - 151 - 162
PY  - 2026
AB  - Laser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples.
KW  - Laser Capture Microdissection: methods
KW  - Chromatin: genetics
KW  - Chromatin Immunoprecipitation Sequencing: methods
KW  - Humans
KW  - High-Throughput Nucleotide Sequencing: methods
KW  - ATAC-seq (Other)
KW  - Chromatin accessibility (Other)
KW  - Laser-capture microdissection (Other)
KW  - Morphological staining (Other)
KW  - Spatial omics (Other)
KW  - Chromatin (NLM Chemicals)
LB  - PUB:(DE-HGF)7
C6  - pmid:41629717
DO  - DOI:10.1007/978-1-0716-5154-4_12
UR  - https://pub.dzne.de/record/285026
ER  -