Home > In process > In vivo CRISPR screen reveals regulation of macrophage states in neuroinflammation. > print

001     285047
005     20260209103813.0
024 7 _ |a 10.1038/s41593-025-02151-6
|2 doi
024 7 _ |a pmid:41345278
|2 pmid
024 7 _ |a pmc:PMC12880918
|2 pmc
024 7 _ |a 1097-6256
|2 ISSN
024 7 _ |a 1546-1726
|2 ISSN
037 _ _ |a DZNE-2026-00171
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a de la Rosa, Clara
|0 0000-0002-1344-0844
|b 0
245 _ _ |a In vivo CRISPR screen reveals regulation of macrophage states in neuroinflammation.
260 _ _ |a New York, NY
|c 2026
|b Nature America
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1770629744_24271
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Here we established an in vivo CRISPR screening pipeline using genetically editable progenitor cells to dissect macrophage regulation in mouse models of multiple sclerosis (MS). Screening over 100 cytokine receptors and signaling molecules identified interferon-γ, tumor necrosis factor, granulocyte-macrophage colony-stimulating factor and transforming growth factor-β as essential regulators of macrophage polarization in vivo. Single-cell transcriptomics confirmed that transferred progenitor cells generate all blood-derived CNS myeloid cell populations, enabling Perturb-seq analysis of cytokine actions in neuroinflammation. Combined with biosensor expression, our approach allows monitoring cytokine effects on myeloid cell migration, debris phagocytosis and oxidative activity in vivo. Comparative transcriptomic analyses revealed conserved neuroinflammatory cytokine signatures across myeloid populations, CNS compartments and species, elucidating cytokine cues shaping myeloid function in the cerebrospinal fluid and parenchyma of individuals with MS. This versatile pipeline thus provides a scalable framework for high-resolution analysis of macrophage states and uncovers the cytokine signals that underlie their regulation in MS and MS models.
536 _ _ |a 351 - Brain Function (POF4-351)
|0 G:(DE-HGF)POF4-351
|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a Cytokines
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Macrophages: metabolism
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Cytokines: metabolism
|2 MeSH
650 _ 2 |a Cytokines: genetics
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Clustered Regularly Interspaced Short Palindromic Repeats
|2 MeSH
650 _ 2 |a Multiple Sclerosis: immunology
|2 MeSH
650 _ 2 |a Multiple Sclerosis: genetics
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Neuroinflammatory Diseases: genetics
|2 MeSH
650 _ 2 |a CRISPR-Cas Systems
|2 MeSH
650 _ 2 |a Encephalomyelitis, Autoimmune, Experimental: immunology
|2 MeSH
700 1 _ |a Kendirli, Arek
|0 0000-0001-6888-9131
|b 1
700 1 _ |a Baygün, Seren
|b 2
700 1 _ |a Bauernschmitt, Franz
|b 3
700 1 _ |a Thomann, Anna S
|0 0000-0001-6934-8839
|b 4
700 1 _ |a Kisioglu, Ilgin
|b 5
700 1 _ |a Beckmann, Daniela
|b 6
700 1 _ |a Carpentier Solorio, Yves
|b 7
700 1 _ |a Pfaffenstaller, Veronika
|b 8
700 1 _ |a Tai, Yi-Heng
|b 9
700 1 _ |a Mehraein, Niel
|b 10
700 1 _ |a Sanchez, Paula
|b 11
700 1 _ |a Spieth, Lena
|0 P:(DE-2719)9002306
|b 12
|u dzne
700 1 _ |a Gerdes, Lisa Ann
|b 13
700 1 _ |a Beltran, Eduardo
|0 0000-0002-7266-4098
|b 14
700 1 _ |a Dornmair, Klaus
|0 0000-0003-0342-5373
|b 15
700 1 _ |a Simons, Mikael
|0 P:(DE-2719)2811642
|b 16
700 1 _ |a Peters, Anneli
|0 0000-0002-8259-2219
|b 17
700 1 _ |a Schmidt-Supprian, Marc
|0 0000-0002-8543-6166
|b 18
700 1 _ |a Kerschensteiner, Martin
|0 0000-0003-4898-9383
|b 19
773 _ _ |a 10.1038/s41593-025-02151-6
|g Vol. 29, no. 2, p. 493 - 509
|0 PERI:(DE-600)1494955-6
|n 2
|p 493 - 509
|t Nature neuroscience
|v 29
|y 2026
|x 1097-6256
856 4 _ |u https://pub.dzne.de/record/285047/files/DZNE-2026-00171.pdf
|y Restricted
856 4 _ |u https://pub.dzne.de/record/285047/files/DZNE-2026-00171.pdf?subformat=pdfa
|x pdfa
|y Restricted
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 12
|6 P:(DE-2719)9002306
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 16
|6 P:(DE-2719)2811642
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-351
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Brain Function
|x 0
915 _ _ |a National-Konsortium
|0 StatID:(DE-HGF)0430
|2 StatID
|d 2025-11-07
|w ger
915 _ _ |a DEAL Nature
|0 StatID:(DE-HGF)3003
|2 StatID
|d 2025-11-07
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2025-11-07
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2025-11-07
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b NAT NEUROSCI : 2022
|d 2025-11-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2025-11-07
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2025-11-07
915 _ _ |a IF >= 25
|0 StatID:(DE-HGF)9925
|2 StatID
|b NAT NEUROSCI : 2022
|d 2025-11-07
920 1 _ |0 I:(DE-2719)1110008
|k AG Simons
|l Molecular Neurobiology
|x 0
980 _ _ |a journal
980 _ _ |a EDITORS
980 _ _ |a VDBINPRINT
980 _ _ |a I:(DE-2719)1110008
980 _ _ |a UNRESTRICTED

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21