%0 Journal Article
%A Toda Robert, Aiko
%A McQuade, Amanda
%A Koppes-den Hertog, Sascha J
%A Erlebach, Lena
%A Kronenberg-Versteeg, Deborah
%A Kampmann, Martin
%A Giera, Martin
%A van der Kant, Rik
%T Comparative lipidomics of iPSC-derived microglia protocols reveal lipid droplet and immune differences mediated by media composition.
%J Stem cell reports
%V 21
%N 2
%@ 2213-6711
%C Maryland Heights, MO
%I Cell Press
%M DZNE-2026-00189
%P 102779
%D 2026
%X Altered microglial lipid metabolism is heavily implicated in Alzheimer's disease (AD) and aging. Recently, protocols were developed to generate human induced pluripotent stem cell-derived microglia-like cells (iMGL) to study microglial function in vitro, including embryoid body-based methods and induced transcription factor (iTF)-dependent approaches. Here, we performed comparative lipidomics on iMGL from these methods and report major differences in multiple lipid classes, including triglycerides (TGs), a storage form of fatty acids implicated in microglial reactivity. TGs are strongly increased in iTF microglia due to the absence of a media supplement (B-27). Supplementing iTF microglia with B-27, or its component L-carnitine, reduces TGs and promotes a homeostatic state. B-27 also renders iTF microglia metabolically responsive to immune stimuli. Overall, our data show that iMGL differentiation methods have a major impact on microglial lipidomes and warrant attention when studying AD and neuroinflammatory processes involving lipids.
%K Microglia: metabolism
%K Microglia: cytology
%K Microglia: drug effects
%K Microglia: immunology
%K Induced Pluripotent Stem Cells: cytology
%K Induced Pluripotent Stem Cells: metabolism
%K Induced Pluripotent Stem Cells: drug effects
%K Humans
%K Lipidomics: methods
%K Lipid Droplets: metabolism
%K Culture Media: chemistry
%K Culture Media: pharmacology
%K Cell Differentiation: drug effects
%K Lipid Metabolism
%K Triglycerides: metabolism
%K Cells, Cultured
%K iPSC (Other)
%K lipid droplet (Other)
%K lipid metabolism (Other)
%K lipidomics (Other)
%K microglia (Other)
%K neuroinflammation (Other)
%K triglycerides (Other)
%K Culture Media (NLM Chemicals)
%K Triglycerides (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41512865
%R 10.1016/j.stemcr.2025.102779
%U https://pub.dzne.de/record/285210