000285210 001__ 285210
000285210 005__ 20260212151610.0
000285210 0247_ $$2doi$$a10.1016/j.stemcr.2025.102779
000285210 0247_ $$2pmid$$apmid:41512865
000285210 037__ $$aDZNE-2026-00189
000285210 041__ $$aEnglish
000285210 082__ $$a610
000285210 1001_ $$aToda Robert, Aiko$$b0
000285210 245__ $$aComparative lipidomics of iPSC-derived microglia protocols reveal lipid droplet and immune differences mediated by media composition.
000285210 260__ $$aMaryland Heights, MO$$bCell Press$$c2026
000285210 3367_ $$2DRIVER$$aarticle
000285210 3367_ $$2DataCite$$aOutput Types/Journal article
000285210 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1770905614_9056
000285210 3367_ $$2BibTeX$$aARTICLE
000285210 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000285210 3367_ $$00$$2EndNote$$aJournal Article
000285210 520__ $$aAltered microglial lipid metabolism is heavily implicated in Alzheimer's disease (AD) and aging. Recently, protocols were developed to generate human induced pluripotent stem cell-derived microglia-like cells (iMGL) to study microglial function in vitro, including embryoid body-based methods and induced transcription factor (iTF)-dependent approaches. Here, we performed comparative lipidomics on iMGL from these methods and report major differences in multiple lipid classes, including triglycerides (TGs), a storage form of fatty acids implicated in microglial reactivity. TGs are strongly increased in iTF microglia due to the absence of a media supplement (B-27). Supplementing iTF microglia with B-27, or its component L-carnitine, reduces TGs and promotes a homeostatic state. B-27 also renders iTF microglia metabolically responsive to immune stimuli. Overall, our data show that iMGL differentiation methods have a major impact on microglial lipidomes and warrant attention when studying AD and neuroinflammatory processes involving lipids.
000285210 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000285210 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x1
000285210 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000285210 650_7 $$2Other$$aiPSC
000285210 650_7 $$2Other$$alipid droplet
000285210 650_7 $$2Other$$alipid metabolism
000285210 650_7 $$2Other$$alipidomics
000285210 650_7 $$2Other$$amicroglia
000285210 650_7 $$2Other$$aneuroinflammation
000285210 650_7 $$2Other$$atriglycerides
000285210 650_7 $$2NLM Chemicals$$aCulture Media
000285210 650_7 $$2NLM Chemicals$$aTriglycerides
000285210 650_2 $$2MeSH$$aMicroglia: metabolism
000285210 650_2 $$2MeSH$$aMicroglia: cytology
000285210 650_2 $$2MeSH$$aMicroglia: drug effects
000285210 650_2 $$2MeSH$$aMicroglia: immunology
000285210 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells: cytology
000285210 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells: metabolism
000285210 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells: drug effects
000285210 650_2 $$2MeSH$$aHumans
000285210 650_2 $$2MeSH$$aLipidomics: methods
000285210 650_2 $$2MeSH$$aLipid Droplets: metabolism
000285210 650_2 $$2MeSH$$aCulture Media: chemistry
000285210 650_2 $$2MeSH$$aCulture Media: pharmacology
000285210 650_2 $$2MeSH$$aCell Differentiation: drug effects
000285210 650_2 $$2MeSH$$aLipid Metabolism
000285210 650_2 $$2MeSH$$aTriglycerides: metabolism
000285210 650_2 $$2MeSH$$aCells, Cultured
000285210 7001_ $$aMcQuade, Amanda$$b1
000285210 7001_ $$aKoppes-den Hertog, Sascha J$$b2
000285210 7001_ $$0P:(DE-2719)9000542$$aErlebach, Lena$$b3$$udzne
000285210 7001_ $$0P:(DE-2719)9001451$$aKronenberg-Versteeg, Deborah$$b4$$udzne
000285210 7001_ $$aKampmann, Martin$$b5
000285210 7001_ $$aGiera, Martin$$b6
000285210 7001_ $$avan der Kant, Rik$$b7
000285210 773__ $$0PERI:(DE-600)2720528-9$$a10.1016/j.stemcr.2025.102779$$gVol. 21, no. 2, p. 102779 -$$n2$$p102779$$tStem cell reports$$v21$$x2213-6711$$y2026
000285210 8564_ $$uhttps://pub.dzne.de/record/285210/files/DZNE-2026-00189.pdf$$yRestricted
000285210 8564_ $$uhttps://pub.dzne.de/record/285210/files/DZNE-2026-00189.pdf?subformat=pdfa$$xpdfa$$yRestricted
000285210 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000542$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000285210 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001451$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
000285210 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000285210 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x1
000285210 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSTEM CELL REP : 2022$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-05-02T08:50:39Z
000285210 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-05-02T08:50:39Z
000285210 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2023-05-02T08:50:39Z
000285210 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bSTEM CELL REP : 2022$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2025-01-07
000285210 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2025-01-07
000285210 9201_ $$0I:(DE-2719)1210001$$kAG Jucker$$lCell Biology of Neurological Diseases$$x0
000285210 9201_ $$0I:(DE-2719)1210015$$kAG Kronenberg-Versteeg$$lGlial Cell Biology$$x1
000285210 980__ $$ajournal
000285210 980__ $$aEDITORS
000285210 980__ $$aVDBINPRINT
000285210 980__ $$aI:(DE-2719)1210001
000285210 980__ $$aI:(DE-2719)1210015
000285210 980__ $$aUNRESTRICTED