Home > In process > Comparative lipidomics of iPSC-derived microglia protocols reveal lipid droplet and immune differences mediated by media composition. > print

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024 7 _ |a 10.1016/j.stemcr.2025.102779
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037 _ _ |a DZNE-2026-00189
041 _ _ |a English
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100 1 _ |a Toda Robert, Aiko
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245 _ _ |a Comparative lipidomics of iPSC-derived microglia protocols reveal lipid droplet and immune differences mediated by media composition.
260 _ _ |a Maryland Heights, MO
|c 2026
|b Cell Press
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520 _ _ |a Altered microglial lipid metabolism is heavily implicated in Alzheimer's disease (AD) and aging. Recently, protocols were developed to generate human induced pluripotent stem cell-derived microglia-like cells (iMGL) to study microglial function in vitro, including embryoid body-based methods and induced transcription factor (iTF)-dependent approaches. Here, we performed comparative lipidomics on iMGL from these methods and report major differences in multiple lipid classes, including triglycerides (TGs), a storage form of fatty acids implicated in microglial reactivity. TGs are strongly increased in iTF microglia due to the absence of a media supplement (B-27). Supplementing iTF microglia with B-27, or its component L-carnitine, reduces TGs and promotes a homeostatic state. B-27 also renders iTF microglia metabolically responsive to immune stimuli. Overall, our data show that iMGL differentiation methods have a major impact on microglial lipidomes and warrant attention when studying AD and neuroinflammatory processes involving lipids.
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650 _ 7 |a iPSC
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650 _ 7 |a lipid droplet
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650 _ 7 |a lipid metabolism
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650 _ 7 |a lipidomics
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650 _ 7 |a microglia
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650 _ 7 |a neuroinflammation
|2 Other
650 _ 7 |a triglycerides
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650 _ 7 |a Culture Media
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650 _ 7 |a Triglycerides
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650 _ 2 |a Microglia: metabolism
|2 MeSH
650 _ 2 |a Microglia: cytology
|2 MeSH
650 _ 2 |a Microglia: drug effects
|2 MeSH
650 _ 2 |a Microglia: immunology
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: cytology
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: metabolism
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: drug effects
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Lipidomics: methods
|2 MeSH
650 _ 2 |a Lipid Droplets: metabolism
|2 MeSH
650 _ 2 |a Culture Media: chemistry
|2 MeSH
650 _ 2 |a Culture Media: pharmacology
|2 MeSH
650 _ 2 |a Cell Differentiation: drug effects
|2 MeSH
650 _ 2 |a Lipid Metabolism
|2 MeSH
650 _ 2 |a Triglycerides: metabolism
|2 MeSH
650 _ 2 |a Cells, Cultured
|2 MeSH
700 1 _ |a McQuade, Amanda
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700 1 _ |a Koppes-den Hertog, Sascha J
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700 1 _ |a Erlebach, Lena
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700 1 _ |a Kronenberg-Versteeg, Deborah
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700 1 _ |a Kampmann, Martin
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700 1 _ |a Giera, Martin
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700 1 _ |a van der Kant, Rik
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773 _ _ |a 10.1016/j.stemcr.2025.102779
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