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@ARTICLE{Vlchez:285254,
      author       = {Vílchez, S. and Miras, J. and Farfan, S. and Tur Guasch,
                      Rafael and de Oliveira, N. and Pérez-Calm, A. and Grijalvo,
                      S. and Rodríguez-Abreu, C. and Esquena, J.},
      title        = {{M}icron-sized {DNA}-gelatin coacervates generated by ionic
                      complexation in the presence of a nonionic polysaccharide.},
      journal      = {Journal of colloid and interface science},
      volume       = {711},
      issn         = {0021-9797},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DZNE-2026-00196},
      pages        = {140031},
      year         = {2026},
      abstract     = {DNA-protein coacervate microparticles can be formed via
                      ionic complexation between DNA and a protein, facilitated by
                      the presence of a nonionic polymer. Despite recent advances
                      in understanding membraneless organelles (MLOs) in
                      eukaryotic cells, their formation through liquid-liquid
                      phase separation remains incompletely elucidated. We
                      hypothesized that due to their opposite charges, DNA and
                      gelatin readily form micron-sized coacervates, and particle
                      formation is facilitated by adding a polymer immiscible with
                      gelatin.Formation of coacervate microparticles was essayed
                      in the model system composed of an anionic protein
                      (gelatin), a nonionic polysaccharide (dextran) and DNA from
                      salmon testes. The gelatin-dextran system was chosen because
                      these biopolymers exhibit a broad immiscibility region in
                      their phase diagram, and can form water-in-water emulsions.
                      Particle size was studied as a function of composition
                      parameters, and molecular interactions were evaluated by
                      rheology.Microparticles mainly composed of gelatin and DNA
                      were successfully synthesized, while dextran remained
                      predominantly in the continuous phase. Particle formation
                      was driven by electrostatic interactions between positively
                      charged gelatin and negatively charged DNA, further
                      facilitated by the immiscibility between gelatin and
                      dextran. Rheological analyses confirmed that these spherical
                      particles are indeed microgels, exhibiting high viscosity,
                      pseudoplastic behavior and significant cohesive energy,
                      driven by electrostatic gelatin-DNA interactions.
                      Additionally, particle size could be finely tuned by
                      adjusting the concentrations of the biopolymers.},
      keywords     = {Coacervates (Other) / DNA (Other) / Dextran (Other) /
                      Gelatin (Other) / Liquid-liquid phase separation (Other) /
                      Microgels (Other)},
      cin          = {AG Rodriguez-Muela},
      ddc          = {540},
      cid          = {I:(DE-2719)1713001},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41678882},
      doi          = {10.1016/j.jcis.2026.140031},
      url          = {https://pub.dzne.de/record/285254},
}