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@ARTICLE{Huber:285353,
      author       = {Huber, Hanna and Montoliu-Gaya, Laia and Brum, Wagner S and
                      Vávra, Jakub and Yakoub, Yara and Weninger, Haley and
                      Braun-Wohlfahrt, Luisa Sophie and Simrén, Joel and Boada,
                      Mercé and Ruiz, Agustín and Cano, Amanda and Orellana,
                      Adelina and Valero, Sergi and Cañada, Laia and Tantinya,
                      Natalia and Nogales, Ana Belen and Sanz-Cartagena, Pilar and
                      Dittrich, Anna and Skoog, Ingmar and Sander-Long, Millie and
                      Ballard, Clive and Richards, Megan and O'Leary, Mary and
                      Clemmensen, Frederikke Kragh and Wandall, Hannah H D and
                      Altomare, Daniele and Cantoni, Valentina and Stomrud, Erik
                      and Palmqvist, Sebastian and Lleo, Alberto and Alcolea,
                      Daniel and Carmona Iragui, Maria and Hernandez, Aida Sanjuan
                      and Benejam, Bessy and Videla Toro, Laura and Singh, Alpana
                      and Denkinger, Marisa N and Simonsen, Anja Hviid and Kern,
                      Silke and Corbett, Anne and Fortea, Juan and Honigberg, Lee
                      and Borroni, Barbara and Hansson, Oskar and Morató, Xavier
                      and Blennow, Kaj and Zetterberg, Henrik and Ashton, Nicholas
                      J},
      title        = {{A} minimally invasive dried blood spot biomarker test for
                      the detection of {A}lzheimer's disease pathology.},
      journal      = {Nature medicine},
      volume       = {32},
      number       = {2},
      issn         = {1078-8956},
      address      = {[New York, NY]},
      publisher    = {Springer Nature},
      reportid     = {DZNE-2026-00219},
      pages        = {599 - 608},
      year         = {2026},
      abstract     = {Blood biomarkers have emerged as accurate tools for
                      detecting Alzheimer's disease (AD) pathology, offering a
                      minimally invasive alternative to traditional diagnostic
                      methods such as imaging and cerebrospinal fluid (CSF)
                      analysis. Yet, the logistics surrounding venipuncture for
                      blood collection, although considerably simpler than the
                      acquisition of imaging and CSF, require precise processing
                      and storage specific to AD biomarkers that are still guided
                      by medical personnel. Consequently, limitations in their
                      widescale use in research and broader clinical
                      implementation exist. The DROP-AD project investigates the
                      potential of dried plasma spot (DPS) and dried blood spot
                      (DBS) analysis, derived from capillary blood, for detecting
                      AD biomarkers, including phosphorylated tau at amino acid
                      217 (p-tau217), glial fibrillary acidic protein and
                      neurofilament light. Here, 337 participants from 7 centers
                      were included, with 304 participants providing paired
                      capillary DPS or DBS and venous plasma samples. We observed
                      strong correlations between DPS p-tau217 and venous plasma
                      p-tau217 (rS = 0.74, P < 0.001). DPS p-tau217 progressively
                      increased with increasing disease severity, and showed good
                      accuracy in predicting CSF biomarker positivity (area under
                      the curve = 0.864). Similarly, we demonstrated the
                      successful detection of glial fibrillary acidic protein and
                      neurofilament light with strong correlations between DBS and
                      DPS, respectively, using paired venous plasma samples.
                      Notably, the method was also effective in individuals with
                      Down syndrome, a population at high genetic risk for AD but
                      in whom standard blood sampling by venipuncture may be more
                      complicated, revealing elevated biomarkers in those with
                      dementia compared with asymptomatic individuals. The study
                      also explored unsupervised blood collection, finding high
                      concordance between supervised and self-collected samples.
                      These findings underscore the potential of dried blood
                      collection and capillary blood as a minimally invasive,
                      scalable approach for AD biomarker testing in research
                      settings. Yet, further refinement of collection and
                      analytical protocols is needed to fully translate this
                      approach to be viable and useful as a clinical tool.},
      keywords     = {Humans / Alzheimer Disease: blood / Alzheimer Disease:
                      diagnosis / Alzheimer Disease: pathology / Dried Blood Spot
                      Testing: methods / Biomarkers: blood / Biomarkers:
                      cerebrospinal fluid / Female / tau Proteins: blood / Male /
                      Aged / Neurofilament Proteins: blood / Glial Fibrillary
                      Acidic Protein: blood / Aged, 80 and over / Middle Aged /
                      Phosphorylation / Biomarkers (NLM Chemicals) / tau Proteins
                      (NLM Chemicals) / Neurofilament Proteins (NLM Chemicals) /
                      neurofilament protein L (NLM Chemicals) / Glial Fibrillary
                      Acidic Protein (NLM Chemicals) / MAPT protein, human (NLM
                      Chemicals)},
      cin          = {AG Schneider},
      ddc          = {610},
      cid          = {I:(DE-2719)1011305},
      pnm          = {353 - Clinical and Health Care Research (POF4-353)},
      pid          = {G:(DE-HGF)POF4-353},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41491101},
      pmc          = {pmc:PMC12920126},
      doi          = {10.1038/s41591-025-04080-0},
      url          = {https://pub.dzne.de/record/285353},
}