000136114 001__ 136114
000136114 005__ 20250325111223.0
000136114 0247_ $$2doi$$a10.1002/psc.1227
000136114 0247_ $$2pmid$$apmid:20862726
000136114 0247_ $$2ISSN$$a1075-2617
000136114 0247_ $$2ISSN$$a1099-1387
000136114 037__ $$aDZNE-2020-02436
000136114 041__ $$aEnglish
000136114 082__ $$a540
000136114 1001_ $$0P:(DE-HGF)0$$aChu, Nam Ky$$b0
000136114 245__ $$aProtein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing.
000136114 260__ $$aNew York, NY [u.a.]$$bWiley$$c2010
000136114 264_1 $$2Crossref$$3online$$bWiley$$c2010-09-16
000136114 264_1 $$2Crossref$$3print$$bWiley$$c2010-10-01
000136114 3367_ $$2DRIVER$$aarticle
000136114 3367_ $$2DataCite$$aOutput Types/Journal article
000136114 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1742897502_32038
000136114 3367_ $$2BibTeX$$aARTICLE
000136114 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000136114 3367_ $$00$$2EndNote$$aJournal Article
000136114 520__ $$aA plethora of methods exist to link proteins to surfaces in order to generate functionalized materials. However, general tools that lead to functional immobilization of recombinantly expressed proteins on membranes such as liposomes or lipid-coated nanoparticles are rare. Here we present an approach that takes advantage of a double-palmitoylated peptide that mediates stable membrane anchoring in combination with protein trans-splicing for efficient immobilization of recombinant proteins fused to split intein segments. Two different DnaE split inteins from Synechocystis and Nostoc punctiforme are tested and compared to immobilization via direct native chemical ligation using a protein thioester. Protein trans-splicing proceeds at low protein concentrations and leads to functionalized vesicles and membrane-coated silica nanoparticles.
000136114 536__ $$0G:(DE-HGF)POF3-341$$a341 - Molecular Signaling (POF3-341)$$cPOF3-341$$fPOF III$$x0
000136114 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x1
000136114 542__ $$2Crossref$$i2015-09-01$$uhttp://doi.wiley.com/10.1002/tdm_license_1.1
000136114 588__ $$aDataset connected to CrossRef, PubMed,
000136114 650_7 $$2NLM Chemicals$$aImmobilized Proteins
000136114 650_7 $$2NLM Chemicals$$aLipids
000136114 650_7 $$2NLM Chemicals$$aLiposomes
000136114 650_7 $$2NLM Chemicals$$aRecombinant Fusion Proteins
000136114 650_2 $$2MeSH$$aImmobilized Proteins: chemistry
000136114 650_2 $$2MeSH$$aImmobilized Proteins: metabolism
000136114 650_2 $$2MeSH$$aInteins: genetics
000136114 650_2 $$2MeSH$$aLipids: chemistry
000136114 650_2 $$2MeSH$$aLiposomes: metabolism
000136114 650_2 $$2MeSH$$aModels, Molecular
000136114 650_2 $$2MeSH$$aNanoparticles: chemistry
000136114 650_2 $$2MeSH$$aNostoc: genetics
000136114 650_2 $$2MeSH$$aProtein Splicing
000136114 650_2 $$2MeSH$$aRecombinant Fusion Proteins: chemistry
000136114 650_2 $$2MeSH$$aRecombinant Fusion Proteins: genetics
000136114 650_2 $$2MeSH$$aRecombinant Fusion Proteins: metabolism
000136114 650_2 $$2MeSH$$aSynechocystis: genetics
000136114 650_2 $$2MeSH$$aTrans-Splicing
000136114 7001_ $$0P:(DE-HGF)0$$aOlschewski, Diana$$b1
000136114 7001_ $$0P:(DE-HGF)0$$aSeidel, Ralf$$b2
000136114 7001_ $$0P:(DE-2719)9000369$$aWinklhofer, Konstanze F$$b3
000136114 7001_ $$0P:(DE-2719)9000396$$aTatzelt, Jörg$$b4$$udzne
000136114 7001_ $$0P:(DE-HGF)0$$aEngelhard, Martin$$b5
000136114 7001_ $$0P:(DE-HGF)0$$aBecker, Christian F W$$b6$$eCorresponding author
000136114 770__ $$aProbing Protein Function through Chemistry
000136114 77318 $$2Crossref$$3journal-article$$a10.1002/psc.1227$$b : Wiley, 2010-09-16$$n10$$p582-588$$tJournal of Peptide Science$$v16$$x1075-2617$$y2010
000136114 773__ $$0PERI:(DE-600)1491819-5$$a10.1002/psc.1227$$gVol. 16, no. 10, p. 582 - 588$$n10$$p582-588$$q16:10<582 - 588$$tJournal of peptide science$$v16$$x1075-2617$$y2010
000136114 8564_ $$uhttps://pub.dzne.de/record/136114/files/DZNE-2020-02436_Restricted.pdf
000136114 8564_ $$uhttps://pub.dzne.de/record/136114/files/DZNE-2020-02436_Restricted.pdf?subformat=pdfa$$xpdfa
000136114 909CO $$ooai:pub.dzne.de:136114$$pVDB
000136114 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000369$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000136114 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000396$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
000136114 9131_ $$0G:(DE-HGF)POF3-341$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vMolecular Signaling$$x0
000136114 9131_ $$0G:(DE-HGF)POF3-342$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vDisease Mechanisms and Model Systems$$x1
000136114 9141_ $$y2010
000136114 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000136114 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000136114 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000136114 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000136114 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bJ PEPT SCI : 2017
000136114 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000136114 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000136114 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000136114 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000136114 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000136114 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000136114 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000136114 9201_ $$0I:(DE-2719)6000016$$kMünchen common$$lMünchen common$$x0
000136114 980__ $$ajournal
000136114 980__ $$aVDB
000136114 980__ $$aI:(DE-2719)6000016
000136114 980__ $$aUNRESTRICTED
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1126/science.1105654$$o10.1126/science.1105654
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1002/(SICI)1521-3765(19990401)5:4<1239::AID-CHEM1239>3.0.CO;2-N$$o10.1002/(SICI)1521-3765(19990401)5:4<1239::AID-CHEM1239>3.0.CO;2-N
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1002/anie.200600855$$o10.1002/anie.200600855
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja052407f$$o10.1021/ja052407f
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1111/j.1742-4658.2006.05152.x$$o10.1111/j.1742-4658.2006.05152.x
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/bc0602782$$o10.1021/bc0602782
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1126/science.7973629$$o10.1126/science.7973629
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nrd1632$$o10.1038/nrd1632
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nmeth886$$o10.1038/nmeth886
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.bioeng.2005.07.004$$o10.1016/j.bioeng.2005.07.004
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja0456611$$o10.1021/ja0456611
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1002/anie.200503475$$o10.1002/anie.200503475
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1111/j.1399-3011.1992.tb00291.x$$o10.1111/j.1399-3011.1992.tb00291.x
000136114 999C5 $$2Crossref$$oAtherton Solid Phase Peptide Synthesis: A Practical Approach. 1989$$tSolid Phase Peptide Synthesis: A Practical Approach.$$y1989
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.275.13.9091$$o10.1074/jbc.275.13.9091
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.febslet.2006.02.045$$o10.1016/j.febslet.2006.02.045
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja9909879$$o10.1021/ja9909879
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M313212200$$o10.1074/jbc.M313212200
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/bi0494065$$o10.1021/bi0494065
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja034736i$$o10.1021/ja034736i
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja042287w$$o10.1021/ja042287w
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/bi001786g$$o10.1021/bi001786g
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.febslet.2009.02.003$$o10.1016/j.febslet.2009.02.003
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0005185$$o10.1371/journal.pone.0005185
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1002/anie.200600570$$o10.1002/anie.200600570
000136114 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1002/bit.21912$$o10.1002/bit.21912