Journal Article

DZNE-2020-02436

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing.

Chu, N. K. ; Olschewski, D. ; Seidel, R. ; Winklhofer, K. F.DZNE* ; Tatzelt, J.DZNE* ; Engelhard, M. ; Becker, C. F. W. (Corresponding author)

2010
Wiley New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1002/psc.1227

Abstract: A plethora of methods exist to link proteins to surfaces in order to generate functionalized materials. However, general tools that lead to functional immobilization of recombinantly expressed proteins on membranes such as liposomes or lipid-coated nanoparticles are rare. Here we present an approach that takes advantage of a double-palmitoylated peptide that mediates stable membrane anchoring in combination with protein trans-splicing for efficient immobilization of recombinant proteins fused to split intein segments. Two different DnaE split inteins from Synechocystis and Nostoc punctiforme are tested and compared to immobilization via direct native chemical ligation using a protein thioester. Protein trans-splicing proceeds at low protein concentrations and leads to functionalized vesicles and membrane-coated silica nanoparticles.

Keyword(s): Immobilized Proteins: chemistry (MeSH) ; Immobilized Proteins: metabolism (MeSH) ; Inteins: genetics (MeSH) ; Lipids: chemistry (MeSH) ; Liposomes: metabolism (MeSH) ; Models, Molecular (MeSH) ; Nanoparticles: chemistry (MeSH) ; Nostoc: genetics (MeSH) ; Protein Splicing (MeSH) ; Recombinant Fusion Proteins: chemistry (MeSH) ; Recombinant Fusion Proteins: genetics (MeSH) ; Recombinant Fusion Proteins: metabolism (MeSH) ; Synechocystis: genetics (MeSH) ; Trans-Splicing (MeSH) ; Immobilized Proteins ; Lipids ; Liposomes ; Recombinant Fusion Proteins

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Contributing Institute(s):

1. München common (München common)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)
2. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2010

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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