TY  - JOUR
AU  - Chu, Nam Ky
AU  - Olschewski, Diana
AU  - Seidel, Ralf
AU  - Winklhofer, Konstanze F
AU  - Tatzelt, Jörg
AU  - Engelhard, Martin
AU  - Becker, Christian F W
TI  - Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing.
JO  - Journal of peptide science
VL  - 16
IS  - 10
SN  - 1075-2617
CY  - New York, NY [u.a.]
PB  - Wiley
M1  - DZNE-2020-02436
SP  - 582-588
PY  - 2010
AB  - A plethora of methods exist to link proteins to surfaces in order to generate functionalized materials. However, general tools that lead to functional immobilization of recombinantly expressed proteins on membranes such as liposomes or lipid-coated nanoparticles are rare. Here we present an approach that takes advantage of a double-palmitoylated peptide that mediates stable membrane anchoring in combination with protein trans-splicing for efficient immobilization of recombinant proteins fused to split intein segments. Two different DnaE split inteins from Synechocystis and Nostoc punctiforme are tested and compared to immobilization via direct native chemical ligation using a protein thioester. Protein trans-splicing proceeds at low protein concentrations and leads to functionalized vesicles and membrane-coated silica nanoparticles.
KW  - Immobilized Proteins: chemistry
KW  - Immobilized Proteins: metabolism
KW  - Inteins: genetics
KW  - Lipids: chemistry
KW  - Liposomes: metabolism
KW  - Models, Molecular
KW  - Nanoparticles: chemistry
KW  - Nostoc: genetics
KW  - Protein Splicing
KW  - Recombinant Fusion Proteins: chemistry
KW  - Recombinant Fusion Proteins: genetics
KW  - Recombinant Fusion Proteins: metabolism
KW  - Synechocystis: genetics
KW  - Trans-Splicing
KW  - Immobilized Proteins (NLM Chemicals)
KW  - Lipids (NLM Chemicals)
KW  - Liposomes (NLM Chemicals)
KW  - Recombinant Fusion Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:20862726
DO  - DOI:10.1002/psc.1227
UR  - https://pub.dzne.de/record/136114
ER  -