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| Home > Publications Database > Three-dimensional imaging of solvent-cleared organs using 3DISCO. |
| Home > Publications Database > Three-dimensional imaging of solvent-cleared organs using 3DISCO. |
| Journal Article | DZNE-2020-03001 |
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2012
Nature Publishing Group
Basingstoke
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Please use a persistent id in citations: doi:10.1038/nprot.2012.119
Abstract: The examination of tissue histology by light microscopy is a fundamental tool for investigating the structure and function of organs under normal and disease states. Many current techniques for tissue sectioning, imaging and analysis are time-consuming, and they present major limitations for 3D tissue reconstruction. The introduction of methods to achieve the optical clearing and subsequent light-sheet laser scanning of entire transparent organs without sectioning represents a major advance in the field. We recently developed a highly reproducible and versatile clearing procedure called 3D imaging of solvent-cleared organs, or 3DISCO, which is applicable to diverse tissues including brain, spinal cord, immune organs and tumors. Here we describe a detailed protocol for performing 3DISCO and present its application to various microscopy techniques, including example results from various mouse tissues. The tissue clearing takes as little as 3 h, and imaging can be completed in ∼45 min. 3DISCO is a powerful technique that offers 3D histological views of tissues in a fraction of the time and labor required to complete standard histology studies.
Keyword(s): Animals (MeSH) ; Brain: anatomy & histology (MeSH) ; Brain: blood supply (MeSH) ; Furans: chemistry (MeSH) ; Green Fluorescent Proteins: analysis (MeSH) ; Green Fluorescent Proteins: chemistry (MeSH) ; Half-Life (MeSH) ; Imaging, Three-Dimensional: methods (MeSH) ; Mice (MeSH) ; Mice, Transgenic (MeSH) ; Microscopy, Confocal (MeSH) ; Microscopy, Fluorescence: methods (MeSH) ; Phenyl Ethers: chemistry (MeSH) ; Solvents: chemistry (MeSH) ; Spinal Cord: anatomy & histology (MeSH) ; Spinal Cord: blood supply (MeSH) ; Furans ; Phenyl Ethers ; Solvents ; Green Fluorescent Proteins ; dibenzyl ether ; tetrahydrofuran
; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz
; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection
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