TY  - JOUR
AU  - Laskowski, Claudia J
AU  - Bradke, Frank
TI  - In vivo imaging: a dynamic imaging approach to study spinal cord regeneration.
JO  - Experimental neurology
VL  - 242
SN  - 0014-4886
CY  - Orlando, Fla.
PB  - Academic Press
M1  - DZNE-2020-03237
SP  - 11-17
PY  - 2013
AB  - Upon spinal cord injury, severed axons and the surrounding tissue undergo a series of pathological changes, including retraction of proximal axon ends, degeneration of distal axon ends and formation of a dense fibrotic scar that inhibits regenerative axonal growth. Until recently it was technically challenging to study these dynamic events in the mammalian central nervous system. Here, we describe and discuss the recently established genetic tract tracing approach of in vivo imaging. This technique allows studying acute pathological events following a spinal cord lesion. In addition, the novel development of chronic spinal cord preparations such as the implanted spinal chamber now also enables long-term imaging studies. Hence, in vivo imaging allows the direct observation of acute and chronic dynamic degenerative and regenerative events of individual neurons after traumatic injury in the living animal.
KW  - Animals
KW  - Animals, Genetically Modified
KW  - Axons: physiology
KW  - Green Fluorescent Proteins: genetics
KW  - Green Fluorescent Proteins: metabolism
KW  - Neurons: pathology
KW  - Neurons: physiology
KW  - Nonlinear Dynamics
KW  - Optical Imaging: instrumentation
KW  - Optical Imaging: methods
KW  - Spinal Cord: pathology
KW  - Spinal Cord Injuries: pathology
KW  - Spinal Cord Injuries: physiopathology
KW  - Spinal Cord Regeneration: physiology
KW  - Green Fluorescent Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22836145
DO  - DOI:10.1016/j.expneurol.2012.07.007
UR  - https://pub.dzne.de/record/136915
ER  -