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| 001 | 136981 | ||
| 005 | 20240321220152.0 | ||
| 024 | 7 | _ | |a 10.1038/nbt.2612 |2 doi |
| 024 | 7 | _ | |a pmid:23792630 |2 pmid |
| 024 | 7 | _ | |a 0733-222X |2 ISSN |
| 024 | 7 | _ | |a 1087-0156 |2 ISSN |
| 024 | 7 | _ | |a 1546-1696 |2 ISSN |
| 024 | 7 | _ | |a 2331-3684 |2 ISSN |
| 024 | 7 | _ | |a altmetric:1577822 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-03303 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 660 |
| 100 | 1 | _ | |a Gilleron, Jerome |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape. |
| 260 | _ | _ | |a New York, NY |c 2013 |b Nature America |
| 264 | _ | 1 | |3 online |2 Crossref |b Springer Science and Business Media LLC |c 2013-06-23 |
| 264 | _ | 1 | |3 print |2 Crossref |b Springer Science and Business Media LLC |c 2013-07-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1709895119_26560 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Delivery of short interfering RNAs (siRNAs) remains a key challenge in the development of RNA interference (RNAi) therapeutics. A better understanding of the mechanisms of siRNA cellular uptake, intracellular transport and endosomal release could critically contribute to the improvement of delivery methods. Here we monitored the uptake of lipid nanoparticles (LNPs) loaded with traceable siRNAs in different cell types in vitro and in mouse liver by quantitative fluorescence imaging and electron microscopy. We found that LNPs enter cells by both constitutive and inducible pathways in a cell type-specific manner using clathrin-mediated endocytosis as well as macropinocytosis. By directly detecting colloidal-gold particles conjugated to siRNAs, we estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1-2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics. Our results provide insights into LNP-mediated siRNA delivery that can guide development of the next generation of delivery systems for RNAi therapeutics. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 542 | _ | _ | |i 2013-06-23 |2 Crossref |u http://www.springer.com/tdm |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Lipids |2 NLM Chemicals |
| 650 | _ | 7 | |a RNA, Small Interfering |2 NLM Chemicals |
| 650 | _ | 7 | |a Green Fluorescent Proteins |0 147336-22-9 |2 NLM Chemicals |
| 650 | _ | 7 | |a Gold |0 7440-57-5 |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Endocytosis: genetics |2 MeSH |
| 650 | _ | 2 | |a Gene Transfer Techniques |2 MeSH |
| 650 | _ | 2 | |a Gold: administration & dosage |2 MeSH |
| 650 | _ | 2 | |a Gold: chemistry |2 MeSH |
| 650 | _ | 2 | |a Green Fluorescent Proteins: antagonists & inhibitors |2 MeSH |
| 650 | _ | 2 | |a Green Fluorescent Proteins: genetics |2 MeSH |
| 650 | _ | 2 | |a HeLa Cells |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Lipids: administration & dosage |2 MeSH |
| 650 | _ | 2 | |a Lipids: chemistry |2 MeSH |
| 650 | _ | 2 | |a Lipids: genetics |2 MeSH |
| 650 | _ | 2 | |a Metal Nanoparticles: administration & dosage |2 MeSH |
| 650 | _ | 2 | |a Metal Nanoparticles: chemistry |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Microscopy, Electron |2 MeSH |
| 650 | _ | 2 | |a RNA, Small Interfering: administration & dosage |2 MeSH |
| 650 | _ | 2 | |a RNA, Small Interfering: chemistry |2 MeSH |
| 650 | _ | 2 | |a RNA, Small Interfering: genetics |2 MeSH |
| 700 | 1 | _ | |a Querbes, William |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Zeigerer, Anja |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Borodovsky, Anna |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Marsico, Giovanni |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Schubert, Undine |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Manygoats, Kevin |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Seifert, Sarah |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Andree, Cordula |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Stöter, Martin |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Epstein-Barash, Hila |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a Zhang, Ligang |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Koteliansky, Victor |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Fitzgerald, Kevin |0 P:(DE-HGF)0 |b 13 |
| 700 | 1 | _ | |a Fava, Eugenio |0 P:(DE-2719)2159508 |b 14 |u dzne |
| 700 | 1 | _ | |a Bickle, Marc |0 P:(DE-HGF)0 |b 15 |
| 700 | 1 | _ | |a Kalaidzidis, Yannis |0 P:(DE-HGF)0 |b 16 |
| 700 | 1 | _ | |a Akinc, Akin |0 P:(DE-HGF)0 |b 17 |
| 700 | 1 | _ | |a Maier, Martin |0 P:(DE-HGF)0 |b 18 |
| 700 | 1 | _ | |a Zerial, Marino |0 P:(DE-HGF)0 |b 19 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1038/nbt.2612 |b : Springer Science and Business Media LLC, 2013-06-23 |n 7 |p 638-646 |3 journal-article |2 Crossref |t Nature Biotechnology |v 31 |y 2013 |x 1087-0156 |
| 773 | _ | _ | |a 10.1038/nbt.2612 |g Vol. 31, no. 7, p. 638 - 646 |0 PERI:(DE-600)1494943-X |n 7 |q 31:7<638 - 646 |p 638-646 |t Nature biotechnology |v 31 |y 2013 |x 1087-0156 |
| 856 | 4 | _ | |u https://pub.dzne.de/record/136981/files/DZNE-2020-03303_Restricted.pdf |
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| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 14 |6 P:(DE-2719)2159508 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-342 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Disease Mechanisms and Model Systems |x 0 |
| 914 | 1 | _ | |y 2013 |
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