Journal Article

DZNE-2020-03303

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape.

Gilleron, J. ; Querbes, W. ; Zeigerer, A. ; Borodovsky, A. ; Marsico, G. ; Schubert, U. ; Manygoats, K. ; Seifert, S. ; Andree, C. ; Stöter, M. ; Epstein-Barash, H. ; Zhang, L. ; Koteliansky, V. ; Fitzgerald, K. ; Fava, E.DZNE* ; Bickle, M. ; Kalaidzidis, Y. ; Akinc, A. ; Maier, M. ; Zerial, M. (Corresponding author)

2013
Nature America New York, NY

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Please use a persistent id in citations: doi:10.1038/nbt.2612

Abstract: Delivery of short interfering RNAs (siRNAs) remains a key challenge in the development of RNA interference (RNAi) therapeutics. A better understanding of the mechanisms of siRNA cellular uptake, intracellular transport and endosomal release could critically contribute to the improvement of delivery methods. Here we monitored the uptake of lipid nanoparticles (LNPs) loaded with traceable siRNAs in different cell types in vitro and in mouse liver by quantitative fluorescence imaging and electron microscopy. We found that LNPs enter cells by both constitutive and inducible pathways in a cell type-specific manner using clathrin-mediated endocytosis as well as macropinocytosis. By directly detecting colloidal-gold particles conjugated to siRNAs, we estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1-2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics. Our results provide insights into LNP-mediated siRNA delivery that can guide development of the next generation of delivery systems for RNAi therapeutics.

Keyword(s): Animals (MeSH) ; Endocytosis: genetics (MeSH) ; Gene Transfer Techniques (MeSH) ; Gold: administration & dosage (MeSH) ; Gold: chemistry (MeSH) ; Green Fluorescent Proteins: antagonists & inhibitors (MeSH) ; Green Fluorescent Proteins: genetics (MeSH) ; HeLa Cells (MeSH) ; Humans (MeSH) ; Lipids: administration & dosage (MeSH) ; Lipids: chemistry (MeSH) ; Lipids: genetics (MeSH) ; Metal Nanoparticles: administration & dosage (MeSH) ; Metal Nanoparticles: chemistry (MeSH) ; Mice (MeSH) ; Microscopy, Electron (MeSH) ; RNA, Small Interfering: administration & dosage (MeSH) ; RNA, Small Interfering: chemistry (MeSH) ; RNA, Small Interfering: genetics (MeSH) ; Lipids ; RNA, Small Interfering ; Green Fluorescent Proteins ; Gold

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Contributing Institute(s):

1. Systems Phenomics (AG Fava 1)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2013

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 30 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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