%0 Journal Article
%A Höllerhage, Matthias
%A Rösler, Thomas W
%A Berjas, Magda
%A Luo, Rensheng
%A Tran, Kevin
%A Richards, Kristy M
%A Sabaa-Srur, Armando U
%A Maia, José Guilherme S
%A Moraes, Maria Rosa de
%A Godoy, Helena T
%A Höglinger, Günter U
%A Smith, Robert E
%T Neurotoxicity of Dietary Supplements from Annonaceae Species.
%J International journal of toxicology
%V 34
%N 6
%@ 1091-5818
%C London
%I Sage
%M DZNE-2020-04606
%P 543-550
%D 2015
%X Dietary supplements containing plant materials of Annonaceae species (Annona muricata L., A. squamosa L., A. mucosa JACQ., A. squamosa × cherimola Mabb.) were extracted by hot, pressurized ethyl acetate and analyzed for their effect in vitro on Lund human mesencephalic neurons. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell death was determined by lactate dehydrogenase levels. Three supplements strongly decreased the cell viability at extract concentrations of 1 µg/mL, of which 1 decreased cell viability at 0.1 µg/µL. Also, strong neuronal toxicities of these supplements were found. Cell death was observed at concentrations of 10 µg/mL. The degree of toxicity was comparable to the ones found in Annonaceous fruit extracts. Two fruit pulps of Annonaceae (A. muricata and A. squamosa) showed a reduction in cell viability at lower concentrations. The fruit pulp extract of A. muricata revealed the strongest neurotoxic effect, with 67
%K Annonaceae: toxicity
%K Cell Survival: drug effects
%K Cells, Cultured
%K Dietary Supplements: toxicity
%K Fruit: chemistry
%K Fruit: toxicity
%K Humans
%K L-Lactate Dehydrogenase: analysis
%K L-Lactate Dehydrogenase: metabolism
%K Mesencephalon: cytology
%K Mesencephalon: drug effects
%K Neurons: drug effects
%K Neurotoxicity Syndromes: pathology
%K Plant Extracts: toxicity
%K Seeds: chemistry
%K Seeds: toxicity
%K Tetrazolium Salts
%K Thiazoles
%K Plant Extracts (NLM Chemicals)
%K Tetrazolium Salts (NLM Chemicals)
%K Thiazoles (NLM Chemicals)
%K L-Lactate Dehydrogenase (NLM Chemicals)
%K thiazolyl blue (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26405269
%R 10.1177/1091581815602252
%U https://pub.dzne.de/record/138284