Journal Article

DZNE-2020-04606

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Neurotoxicity of Dietary Supplements from Annonaceae Species.

Höllerhage, M. (First author)DZNE* ; Rösler, T. W.DZNE* ; Berjas, M.DZNE* ; Luo, R. ; Tran, K. ; Richards, K. M. ; Sabaa-Srur, A. U. ; Maia, J. G. S. ; Moraes, M. R. d. ; Godoy, H. T. ; Höglinger, G. U.DZNE* ; Smith, R. E. (Corresponding author)

2015
Sage London

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Please use a persistent id in citations: doi:10.1177/1091581815602252

Abstract: Dietary supplements containing plant materials of Annonaceae species (Annona muricata L., A. squamosa L., A. mucosa JACQ., A. squamosa × cherimola Mabb.) were extracted by hot, pressurized ethyl acetate and analyzed for their effect in vitro on Lund human mesencephalic neurons. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell death was determined by lactate dehydrogenase levels. Three supplements strongly decreased the cell viability at extract concentrations of 1 µg/mL, of which 1 decreased cell viability at 0.1 µg/µL. Also, strong neuronal toxicities of these supplements were found. Cell death was observed at concentrations of 10 µg/mL. The degree of toxicity was comparable to the ones found in Annonaceous fruit extracts. Two fruit pulps of Annonaceae (A. muricata and A. squamosa) showed a reduction in cell viability at lower concentrations. The fruit pulp extract of A. muricata revealed the strongest neurotoxic effect, with 67% cell death at a concentration of 1 µg/mL. A high reduction in cell viability coupled with pronounced cell death was found at 0.1 µg/mL for an Annonaceous seed extract. These results demonstrate that the intake of dietary supplements containing plant material from Annonaceae may be hazardous to health in terms of neurotoxicity.

Keyword(s): Annonaceae: toxicity (MeSH) ; Cell Survival: drug effects (MeSH) ; Cells, Cultured (MeSH) ; Dietary Supplements: toxicity (MeSH) ; Fruit: chemistry (MeSH) ; Fruit: toxicity (MeSH) ; Humans (MeSH) ; L-Lactate Dehydrogenase: analysis (MeSH) ; L-Lactate Dehydrogenase: metabolism (MeSH) ; Mesencephalon: cytology (MeSH) ; Mesencephalon: drug effects (MeSH) ; Neurons: drug effects (MeSH) ; Neurotoxicity Syndromes: pathology (MeSH) ; Plant Extracts: toxicity (MeSH) ; Seeds: chemistry (MeSH) ; Seeds: toxicity (MeSH) ; Tetrazolium Salts (MeSH) ; Thiazoles (MeSH) ; Plant Extracts ; Tetrazolium Salts ; Thiazoles ; L-Lactate Dehydrogenase ; thiazolyl blue

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Contributing Institute(s):

1. Translational Neurodegeneration (AG Höglinger)

Research Program(s):

1. 344 - Clinical and Health Care Research (POF3-344) (POF3-344)

Appears in the scientific report 2015

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Database coverage:
; Allianz-Lizenz ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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