TY  - JOUR
AU  - Höllerhage, Matthias
AU  - Rösler, Thomas W
AU  - Berjas, Magda
AU  - Luo, Rensheng
AU  - Tran, Kevin
AU  - Richards, Kristy M
AU  - Sabaa-Srur, Armando U
AU  - Maia, José Guilherme S
AU  - Moraes, Maria Rosa de
AU  - Godoy, Helena T
AU  - Höglinger, Günter U
AU  - Smith, Robert E
TI  - Neurotoxicity of Dietary Supplements from Annonaceae Species.
JO  - International journal of toxicology
VL  - 34
IS  - 6
SN  - 1091-5818
CY  - London
PB  - Sage
M1  - DZNE-2020-04606
SP  - 543-550
PY  - 2015
AB  - Dietary supplements containing plant materials of Annonaceae species (Annona muricata L., A. squamosa L., A. mucosa JACQ., A. squamosa × cherimola Mabb.) were extracted by hot, pressurized ethyl acetate and analyzed for their effect in vitro on Lund human mesencephalic neurons. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell death was determined by lactate dehydrogenase levels. Three supplements strongly decreased the cell viability at extract concentrations of 1 µg/mL, of which 1 decreased cell viability at 0.1 µg/µL. Also, strong neuronal toxicities of these supplements were found. Cell death was observed at concentrations of 10 µg/mL. The degree of toxicity was comparable to the ones found in Annonaceous fruit extracts. Two fruit pulps of Annonaceae (A. muricata and A. squamosa) showed a reduction in cell viability at lower concentrations. The fruit pulp extract of A. muricata revealed the strongest neurotoxic effect, with 67
KW  - Annonaceae: toxicity
KW  - Cell Survival: drug effects
KW  - Cells, Cultured
KW  - Dietary Supplements: toxicity
KW  - Fruit: chemistry
KW  - Fruit: toxicity
KW  - Humans
KW  - L-Lactate Dehydrogenase: analysis
KW  - L-Lactate Dehydrogenase: metabolism
KW  - Mesencephalon: cytology
KW  - Mesencephalon: drug effects
KW  - Neurons: drug effects
KW  - Neurotoxicity Syndromes: pathology
KW  - Plant Extracts: toxicity
KW  - Seeds: chemistry
KW  - Seeds: toxicity
KW  - Tetrazolium Salts
KW  - Thiazoles
KW  - Plant Extracts (NLM Chemicals)
KW  - Tetrazolium Salts (NLM Chemicals)
KW  - Thiazoles (NLM Chemicals)
KW  - L-Lactate Dehydrogenase (NLM Chemicals)
KW  - thiazolyl blue (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:26405269
DO  - DOI:10.1177/1091581815602252
UR  - https://pub.dzne.de/record/138284
ER  -