%0 Journal Article
%A Castillo-Gomez, Esther
%A Kästner, Anne
%A Steiner, Johann
%A Schneider, Anja
%A Hettling, Bilke
%A Poggi, Giulia
%A Ostehr, Kristin
%A Uhr, Manfred
%A Asif, Abdul R
%A Matzke, Mike
%A Schmidt, Ulrike
%A Pfander, Viktoria
%A Hammer, Christian
%A Schulz, Thomas F
%A Binder, Lutz
%A Stöcker, Winfried
%A Weber, Frank
%A Ehrenreich, Hannelore
%T The brain as immunoprecipitator of serum autoantibodies against N-Methyl-D-aspartate receptor subunit NR1.
%J Annals of neurology
%V 79
%N 1
%@ 0364-5134
%C Hoboken, NJ
%I Wiley-Blackwell
%M DZNE-2020-04715
%P 144-151
%D 2016
%X Autoantibodies (AB) against N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) are highly seroprevalent in health and disease. Symptomatic relevance may arise upon compromised blood-brain barrier (BBB). However, it remained unknown whether circulating NMDAR1 AB appear in the cerebrospinal fluid (CSF). Of n = 271 subjects with CSF-serum pairs, 26 were NMDAR1 AB seropositive, but only 1 was CSF positive. Contrariwise, tetanus AB (non-brain-binding) were present in serum and CSF of all subjects, with CSF levels higher upon BBB dysfunction. Translational mouse experiments proved the hypothesis that the brain acts as an 'immunoprecipitator'; simultaneous injection of NMDAR1 AB and the non-brain-binding green fluorescent protein AB resulted in high detectability of the former in brain and the latter in CSF.
%K Adult
%K Animals
%K Autoantibodies: blood
%K Autoantibodies: cerebrospinal fluid
%K Blood-Brain Barrier: physiopathology
%K Brain: immunology
%K Female
%K Humans
%K Male
%K Mice
%K Mice, Inbred C57BL
%K Middle Aged
%K Multiple Sclerosis: blood
%K Multiple Sclerosis: cerebrospinal fluid
%K Receptors, N-Methyl-D-Aspartate: immunology
%K Seroepidemiologic Studies
%K Autoantibodies (NLM Chemicals)
%K NR1 NMDA receptor (NLM Chemicals)
%K Receptors, N-Methyl-D-Aspartate (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26505629
%R 10.1002/ana.24545
%U https://pub.dzne.de/record/138393