TY  - JOUR
AU  - Castillo-Gomez, Esther
AU  - Kästner, Anne
AU  - Steiner, Johann
AU  - Schneider, Anja
AU  - Hettling, Bilke
AU  - Poggi, Giulia
AU  - Ostehr, Kristin
AU  - Uhr, Manfred
AU  - Asif, Abdul R
AU  - Matzke, Mike
AU  - Schmidt, Ulrike
AU  - Pfander, Viktoria
AU  - Hammer, Christian
AU  - Schulz, Thomas F
AU  - Binder, Lutz
AU  - Stöcker, Winfried
AU  - Weber, Frank
AU  - Ehrenreich, Hannelore
TI  - The brain as immunoprecipitator of serum autoantibodies against N-Methyl-D-aspartate receptor subunit NR1.
JO  - Annals of neurology
VL  - 79
IS  - 1
SN  - 0364-5134
CY  - Hoboken, NJ
PB  - Wiley-Blackwell
M1  - DZNE-2020-04715
SP  - 144-151
PY  - 2016
AB  - Autoantibodies (AB) against N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) are highly seroprevalent in health and disease. Symptomatic relevance may arise upon compromised blood-brain barrier (BBB). However, it remained unknown whether circulating NMDAR1 AB appear in the cerebrospinal fluid (CSF). Of n = 271 subjects with CSF-serum pairs, 26 were NMDAR1 AB seropositive, but only 1 was CSF positive. Contrariwise, tetanus AB (non-brain-binding) were present in serum and CSF of all subjects, with CSF levels higher upon BBB dysfunction. Translational mouse experiments proved the hypothesis that the brain acts as an 'immunoprecipitator'; simultaneous injection of NMDAR1 AB and the non-brain-binding green fluorescent protein AB resulted in high detectability of the former in brain and the latter in CSF.
KW  - Adult
KW  - Animals
KW  - Autoantibodies: blood
KW  - Autoantibodies: cerebrospinal fluid
KW  - Blood-Brain Barrier: physiopathology
KW  - Brain: immunology
KW  - Female
KW  - Humans
KW  - Male
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Middle Aged
KW  - Multiple Sclerosis: blood
KW  - Multiple Sclerosis: cerebrospinal fluid
KW  - Receptors, N-Methyl-D-Aspartate: immunology
KW  - Seroepidemiologic Studies
KW  - Autoantibodies (NLM Chemicals)
KW  - NR1 NMDA receptor (NLM Chemicals)
KW  - Receptors, N-Methyl-D-Aspartate (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:26505629
DO  - DOI:10.1002/ana.24545
UR  - https://pub.dzne.de/record/138393
ER  -