000138489 001__ 138489
000138489 005__ 20240321220441.0
000138489 0247_ $$2doi$$a10.1016/j.stemcr.2016.03.001
000138489 0247_ $$2pmid$$apmid:27050948
000138489 0247_ $$2pmc$$apmc:PMC4834051
000138489 0247_ $$2altmetric$$aaltmetric:6411850
000138489 037__ $$aDZNE-2020-04811
000138489 041__ $$aEnglish
000138489 082__ $$a610
000138489 1001_ $$0P:(DE-2719)2810390$$aVölkner, Manuela$$b0$$eFirst author$$udzne
000138489 245__ $$aRetinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.
000138489 260__ $$a[New York, NY]$$bElsevier$$c2016
000138489 264_1 $$2Crossref$$3print$$bElsevier BV$$c2016-04-01
000138489 3367_ $$2DRIVER$$aarticle
000138489 3367_ $$2DataCite$$aOutput Types/Journal article
000138489 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1710423920_24480
000138489 3367_ $$2BibTeX$$aARTICLE
000138489 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000138489 3367_ $$00$$2EndNote$$aJournal Article
000138489 520__ $$aThe plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis, shows expression in similar cell types in mouse in vivo, and in mouse and human retinal organoids. Early or late Notch signaling inhibition forces cell differentiation, generating organoids enriched with cone or rod photoreceptors, respectively, demonstrating the power of our improved organoid system for future research in stem cell biology and regenerative medicine.
000138489 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000138489 542__ $$2Crossref$$i2016-04-01$$uhttps://www.elsevier.com/tdm/userlicense/1.0/
000138489 542__ $$2Crossref$$i2016-03-03$$uhttp://creativecommons.org/licenses/by-nc-nd/4.0/
000138489 588__ $$aDataset connected to CrossRef, PubMed,
000138489 650_7 $$2NLM Chemicals$$aPAX6 Transcription Factor
000138489 650_2 $$2MeSH$$aAnimals
000138489 650_2 $$2MeSH$$aCell Differentiation: genetics
000138489 650_2 $$2MeSH$$aCells, Cultured
000138489 650_2 $$2MeSH$$aGene Expression Profiling
000138489 650_2 $$2MeSH$$aHuman Embryonic Stem Cells: cytology
000138489 650_2 $$2MeSH$$aHuman Embryonic Stem Cells: metabolism
000138489 650_2 $$2MeSH$$aHumans
000138489 650_2 $$2MeSH$$aMice
000138489 650_2 $$2MeSH$$aMice, Transgenic
000138489 650_2 $$2MeSH$$aMicroscopy, Confocal
000138489 650_2 $$2MeSH$$aMouse Embryonic Stem Cells: cytology
000138489 650_2 $$2MeSH$$aMouse Embryonic Stem Cells: metabolism
000138489 650_2 $$2MeSH$$aOrgan Culture Techniques
000138489 650_2 $$2MeSH$$aOrganogenesis: genetics
000138489 650_2 $$2MeSH$$aOrganoids: cytology
000138489 650_2 $$2MeSH$$aOrganoids: metabolism
000138489 650_2 $$2MeSH$$aPAX6 Transcription Factor: genetics
000138489 650_2 $$2MeSH$$aPAX6 Transcription Factor: metabolism
000138489 650_2 $$2MeSH$$aPluripotent Stem Cells: cytology
000138489 650_2 $$2MeSH$$aPluripotent Stem Cells: metabolism
000138489 650_2 $$2MeSH$$aRetina: cytology
000138489 650_2 $$2MeSH$$aRetina: growth & development
000138489 650_2 $$2MeSH$$aRetina: metabolism
000138489 650_2 $$2MeSH$$aReverse Transcriptase Polymerase Chain Reaction
000138489 7001_ $$0P:(DE-2719)2811545$$aZschätzsch, Marlen$$b1$$udzne
000138489 7001_ $$aRostovskaya, Maria$$b2
000138489 7001_ $$0P:(DE-HGF)0$$aOverall, Rupert W$$b3
000138489 7001_ $$aBusskamp, Volker$$b4
000138489 7001_ $$aAnastassiadis, Konstantinos$$b5
000138489 7001_ $$0P:(DE-2719)2000041$$aKarl, Mike O$$b6$$eLast author$$udzne
000138489 77318 $$2Crossref$$3journal-article$$a10.1016/j.stemcr.2016.03.001$$b : Elsevier BV, 2016-04-01$$n4$$p525-538$$tStem Cell Reports$$v6$$x2213-6711$$y2016
000138489 773__ $$0PERI:(DE-600)2720528-9$$a10.1016/j.stemcr.2016.03.001$$gVol. 6, no. 4, p. 525 - 538$$n4$$p525-538$$q6:4<525 - 538$$tStem cell reports$$v6$$x2213-6711$$y2016
000138489 8564_ $$uhttps://pub.dzne.de/record/138489/files/DZNE-2020-04811.pdf$$yOpenAccess
000138489 8564_ $$uhttps://pub.dzne.de/record/138489/files/DZNE-2020-04811.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000138489 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834051
000138489 909CO $$ooai:pub.dzne.de:138489$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000138489 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810390$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000138489 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811545$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000138489 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000041$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b6$$kDZNE
000138489 9131_ $$0G:(DE-HGF)POF3-342$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vDisease Mechanisms and Model Systems$$x0
000138489 9141_ $$y2016
000138489 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000138489 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000138489 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000138489 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSTEM CELL REP : 2017
000138489 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000138489 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000138489 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000138489 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000138489 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000138489 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review
000138489 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bSTEM CELL REP : 2017
000138489 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000138489 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000138489 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000138489 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000138489 9201_ $$0I:(DE-2719)1710004$$kAG Karl$$lRetina Regeneration and Degeneration$$x0
000138489 9201_ $$0I:(DE-2719)1740003$$kCell Culture Platform$$lCell Culture Platform$$x1
000138489 980__ $$ajournal
000138489 980__ $$aVDB
000138489 980__ $$aUNRESTRICTED
000138489 980__ $$aI:(DE-2719)1710004
000138489 980__ $$aI:(DE-2719)1740003
000138489 9801_ $$aFullTexts