Journal Article

DZNE-2020-04811

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.

Völkner, M. (First author)DZNE* ; Zschätzsch, M.DZNE* ; Rostovskaya, M. ; Overall, R. W. ; Busskamp, V. ; Anastassiadis, K. ; Karl, M. O. (Last author)DZNE*

2016
Elsevier [New York, NY]

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Please use a persistent id in citations: doi:10.1016/j.stemcr.2016.03.001

Abstract: The plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis, shows expression in similar cell types in mouse in vivo, and in mouse and human retinal organoids. Early or late Notch signaling inhibition forces cell differentiation, generating organoids enriched with cone or rod photoreceptors, respectively, demonstrating the power of our improved organoid system for future research in stem cell biology and regenerative medicine.

Keyword(s): Animals (MeSH) ; Cell Differentiation: genetics (MeSH) ; Cells, Cultured (MeSH) ; Gene Expression Profiling (MeSH) ; Human Embryonic Stem Cells: cytology (MeSH) ; Human Embryonic Stem Cells: metabolism (MeSH) ; Humans (MeSH) ; Mice (MeSH) ; Mice, Transgenic (MeSH) ; Microscopy, Confocal (MeSH) ; Mouse Embryonic Stem Cells: cytology (MeSH) ; Mouse Embryonic Stem Cells: metabolism (MeSH) ; Organ Culture Techniques (MeSH) ; Organogenesis: genetics (MeSH) ; Organoids: cytology (MeSH) ; Organoids: metabolism (MeSH) ; PAX6 Transcription Factor: genetics (MeSH) ; PAX6 Transcription Factor: metabolism (MeSH) ; Pluripotent Stem Cells: cytology (MeSH) ; Pluripotent Stem Cells: metabolism (MeSH) ; Retina: cytology (MeSH) ; Retina: growth & development (MeSH) ; Retina: metabolism (MeSH) ; Reverse Transcriptase Polymerase Chain Reaction (MeSH) ; PAX6 Transcription Factor

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Contributing Institute(s):

1. Retina Regeneration and Degeneration (AG Karl)
2. Cell Culture Platform (Cell Culture Platform)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2016

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Database coverage:
; ; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; DOAJ Seal ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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