Home > Publications Database > Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis. > print

001     138489
005     20240321220441.0
024 7 _ |a 10.1016/j.stemcr.2016.03.001
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037 _ _ |a DZNE-2020-04811
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Völkner, Manuela
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245 _ _ |a Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.
260 _ _ |a [New York, NY]
|c 2016
|b Elsevier
264 _ 1 |3 print
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|b Elsevier BV
|c 2016-04-01
336 7 _ |a article
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336 7 _ |a ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a The plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis, shows expression in similar cell types in mouse in vivo, and in mouse and human retinal organoids. Early or late Notch signaling inhibition forces cell differentiation, generating organoids enriched with cone or rod photoreceptors, respectively, demonstrating the power of our improved organoid system for future research in stem cell biology and regenerative medicine.
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542 _ _ |i 2016-04-01
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542 _ _ |i 2016-03-03
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650 _ 7 |a PAX6 Transcription Factor
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Cell Differentiation: genetics
|2 MeSH
650 _ 2 |a Cells, Cultured
|2 MeSH
650 _ 2 |a Gene Expression Profiling
|2 MeSH
650 _ 2 |a Human Embryonic Stem Cells: cytology
|2 MeSH
650 _ 2 |a Human Embryonic Stem Cells: metabolism
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Mice, Transgenic
|2 MeSH
650 _ 2 |a Microscopy, Confocal
|2 MeSH
650 _ 2 |a Mouse Embryonic Stem Cells: cytology
|2 MeSH
650 _ 2 |a Mouse Embryonic Stem Cells: metabolism
|2 MeSH
650 _ 2 |a Organ Culture Techniques
|2 MeSH
650 _ 2 |a Organogenesis: genetics
|2 MeSH
650 _ 2 |a Organoids: cytology
|2 MeSH
650 _ 2 |a Organoids: metabolism
|2 MeSH
650 _ 2 |a PAX6 Transcription Factor: genetics
|2 MeSH
650 _ 2 |a PAX6 Transcription Factor: metabolism
|2 MeSH
650 _ 2 |a Pluripotent Stem Cells: cytology
|2 MeSH
650 _ 2 |a Pluripotent Stem Cells: metabolism
|2 MeSH
650 _ 2 |a Retina: cytology
|2 MeSH
650 _ 2 |a Retina: growth & development
|2 MeSH
650 _ 2 |a Retina: metabolism
|2 MeSH
650 _ 2 |a Reverse Transcriptase Polymerase Chain Reaction
|2 MeSH
700 1 _ |a Zschätzsch, Marlen
|0 P:(DE-2719)2811545
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700 1 _ |a Rostovskaya, Maria
|b 2
700 1 _ |a Overall, Rupert W
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700 1 _ |a Busskamp, Volker
|b 4
700 1 _ |a Anastassiadis, Konstantinos
|b 5
700 1 _ |a Karl, Mike O
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773 1 8 |a 10.1016/j.stemcr.2016.03.001
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|t Stem Cell Reports
|v 6
|y 2016
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773 _ _ |a 10.1016/j.stemcr.2016.03.001
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856 4 _ |y OpenAccess
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