Journal Article

DZNE-2020-04990

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Proteome rearrangements after auditory learning: high-resolution profiling of synapse-enriched protein fractions from mouse brain.

Kähne, T. (Corresponding author) ; Richter, S. ; Kolodziej, A. ; Smalla, K.-H. ; Pielot, R. ; Engler, A. ; Ohl, F. W. ; Dieterich, D. C. ; Seidenbecher, C. ; Tischmeyer, W. ; Naumann, M. ; Gundelfinger, E. (Last author)DZNE*

2016
Wiley-Blackwell Oxford

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Please use a persistent id in citations: doi:10.1111/jnc.13636

Abstract: Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation of individual synaptic proteins during these processes, much less is known about the complex regulation of synaptic proteomes. Recently, we reported that auditory discrimination learning in mice is associated with a relative down-regulation of proteins involved in the structural organization of synapses in various brain regions. Aiming at the identification of biological processes and signaling pathways involved in auditory memory formation, here, a label-free quantification approach was utilized to identify regulated synaptic junctional proteins and phosphoproteins in the auditory cortex, frontal cortex, hippocampus, and striatum of mice 24 h after the learning experiment. Twenty proteins, including postsynaptic scaffolds, actin-remodeling proteins, and RNA-binding proteins, were regulated in at least three brain regions pointing to common, cross-regional mechanisms. Most of the detected synaptic proteome changes were, however, restricted to individual brain regions. For example, several members of the Septin family of cytoskeletal proteins were up-regulated only in the hippocampus, while Septin-9 was down-regulated in the hippocampus, the frontal cortex, and the striatum. Meta analyses utilizing several databases were employed to identify underlying cellular functions and biological pathways. Data are available via ProteomeExchange with identifier PXD003089. How does the protein composition of synapses change in different brain areas upon auditory learning? We unravel discrete proteome changes in mouse auditory cortex, frontal cortex, hippocampus, and striatum functionally implicated in the learning process. We identify not only common but also area-specific biological pathways and cellular processes modulated 24 h after training, indicating individual contributions of the regions to memory processing.

Keyword(s): Acoustic Stimulation (MeSH) ; Animals (MeSH) ; Auditory Pathways: metabolism (MeSH) ; Brain: metabolism (MeSH) ; Cytoskeletal Proteins: metabolism (MeSH) ; Discrimination Learning: physiology (MeSH) ; Gene Expression Regulation: physiology (MeSH) ; Male (MeSH) ; Metabolic Networks and Pathways (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Phosphoproteins: metabolism (MeSH) ; Proteome: metabolism (MeSH) ; Signal Transduction (MeSH) ; Synapses: metabolism (MeSH) ; Cytoskeletal Proteins ; Phosphoproteins ; Proteome

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Contributing Institute(s):

1. U Preclinical Researchers T1 - Magdeburg (U Preclinical Researchers T1 - Magdeburg)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2016

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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