TY  - JOUR
AU  - Marchetti, Giovanni
AU  - Tavosanis, Gaia
TI  - Steroid Hormone Ecdysone Signaling Specifies Mushroom Body Neuron Sequential Fate via Chinmo.
JO  - Current biology
VL  - 27
IS  - 19
SN  - 0960-9822
CY  - London
PB  - Current Biology Ltd.
M1  - DZNE-2020-05870
SP  - 3017-3024.e4
PY  - 2017
AB  - The functional variety in neuronal composition of an adult brain is established during development. Recent studies proposed that interactions between genetic intrinsic programs and external cues are necessary to generate proper neural diversity [1]. However, the molecular mechanisms underlying this developmental process are still poorly understood. Three main subtypes of Drosophila mushroom body (MB) neurons are sequentially generated during development and provide a good example of developmental neural plasticity [2]. Our present data propose that the environmentally controlled steroid hormone ecdysone functions as a regulator of early-born MB neuron fate during larval-pupal transition. We found that the BTB-zinc finger factor Chinmo acts upstream of ecdysone signaling to promote a neuronal fate switch. Indeed, Chinmo regulates the expression of the ecdysone receptor B1 isoform to mediate the production of γ and α'β' MB neurons. In addition, we provide genetic evidence for a regulatory negative feedback loop driving the α'β' to αβ MB neuron transition in which ecdysone signaling in turn controls microRNA let-7 depression of Chinmo expression. Thus, our results uncover a novel interaction in the MB neural specification pathway for temporal control of neuronal identity by interplay between an extrinsic hormonal signal and an intrinsic transcription factor cascade.
KW  - Animals
KW  - Cell Differentiation
KW  - Drosophila Proteins: genetics
KW  - Drosophila Proteins: metabolism
KW  - Drosophila melanogaster: genetics
KW  - Drosophila melanogaster: growth & development
KW  - Drosophila melanogaster: metabolism
KW  - Ecdysone: metabolism
KW  - Gene Expression Regulation, Developmental
KW  - Larva: genetics
KW  - Larva: growth & development
KW  - Larva: metabolism
KW  - Mushroom Bodies: growth & development
KW  - Nerve Tissue Proteins: genetics
KW  - Nerve Tissue Proteins: metabolism
KW  - Neurons: physiology
KW  - Pupa: genetics
KW  - Pupa: growth & development
KW  - Pupa: metabolism
KW  - Receptors, Steroid: genetics
KW  - Receptors, Steroid: metabolism
KW  - Signal Transduction
KW  - Chinmo protein, Drosophila (NLM Chemicals)
KW  - Drosophila Proteins (NLM Chemicals)
KW  - Nerve Tissue Proteins (NLM Chemicals)
KW  - Receptors, Steroid (NLM Chemicals)
KW  - ecdysone receptor (NLM Chemicals)
KW  - Ecdysone (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:28966087
DO  - DOI:10.1016/j.cub.2017.08.037
UR  - https://pub.dzne.de/record/139548
ER  -