%0 Journal Article
%A Chen, Xufeng
%A Aslam, Muhammad
%A Gollisch, Tim
%A Allen, Kevin
%A Engelhardt, Jakob
%T CKAMP44 modulates integration of visual inputs in the lateral geniculate nucleus.
%J Nature Communications
%V 9
%N 1
%@ 2041-1723
%C [London]
%I Nature Publishing Group UK
%M DZNE-2020-06058
%P 261
%D 2018
%X Relay neurons in the dorsal lateral geniculate nucleus (dLGN) receive excitatory inputs from retinal ganglion cells (RGCs). Retinogeniculate synapses are characterized by a prominent short-term depression of AMPA receptor (AMPAR)-mediated currents, but the underlying mechanisms and its function for visual integration are not known. Here we identify CKAMP44 as a crucial auxiliary subunit of AMPARs in dLGN relay neurons, where it increases AMPAR-mediated current amplitudes and modulates gating of AMPARs. Importantly, CKAMP44 is responsible for the distinctive short-term depression in retinogeniculate synapses by reducing the rate of recovery from desensitization of AMPARs. Genetic deletion of CKAMP44 strongly reduces synaptic short-term depression, which leads to increased spike probability of relay neurons when activated with high-frequency inputs from retinogeniculate synapses. Finally, in vivo recordings reveal augmented ON- and OFF-responses of dLGN neurons in CKAMP44 knockout (CKAMP44-/-) mice, demonstrating the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration.
%K Animals
%K Electroretinography
%K Excitatory Postsynaptic Potentials: physiology
%K Female
%K Geniculate Bodies: cytology
%K Geniculate Bodies: metabolism
%K Geniculate Bodies: physiology
%K Mice, Inbred C57BL
%K Mice, Knockout
%K Nerve Tissue Proteins: genetics
%K Nerve Tissue Proteins: metabolism
%K Neurons: metabolism
%K Neurons: physiology
%K Photic Stimulation
%K Receptors, AMPA: metabolism
%K Retina: cytology
%K Retina: metabolism
%K Retina: physiology
%K Synapses: physiology
%K Synaptic Transmission: genetics
%K Synaptic Transmission: physiology
%K CKAMP44 protein, mouse (NLM Chemicals)
%K Nerve Tissue Proteins (NLM Chemicals)
%K Receptors, AMPA (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:29343769
%2 pmc:PMC5772470
%R 10.1038/s41467-017-02415-1
%U https://pub.dzne.de/record/139736