Journal Article

DZNE-2020-06058

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png CKAMP44 modulates integration of visual inputs in the lateral geniculate nucleus.

Chen, X. (First author)DZNE* ; Aslam, M.DZNE* ; Gollisch, T. ; Allen, K. ; Engelhardt, J. (Last author)DZNE*

2018
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-017-02415-1

Abstract: Relay neurons in the dorsal lateral geniculate nucleus (dLGN) receive excitatory inputs from retinal ganglion cells (RGCs). Retinogeniculate synapses are characterized by a prominent short-term depression of AMPA receptor (AMPAR)-mediated currents, but the underlying mechanisms and its function for visual integration are not known. Here we identify CKAMP44 as a crucial auxiliary subunit of AMPARs in dLGN relay neurons, where it increases AMPAR-mediated current amplitudes and modulates gating of AMPARs. Importantly, CKAMP44 is responsible for the distinctive short-term depression in retinogeniculate synapses by reducing the rate of recovery from desensitization of AMPARs. Genetic deletion of CKAMP44 strongly reduces synaptic short-term depression, which leads to increased spike probability of relay neurons when activated with high-frequency inputs from retinogeniculate synapses. Finally, in vivo recordings reveal augmented ON- and OFF-responses of dLGN neurons in CKAMP44 knockout (CKAMP44-/-) mice, demonstrating the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration.

Keyword(s): Animals (MeSH) ; Electroretinography (MeSH) ; Excitatory Postsynaptic Potentials: physiology (MeSH) ; Female (MeSH) ; Geniculate Bodies: cytology (MeSH) ; Geniculate Bodies: metabolism (MeSH) ; Geniculate Bodies: physiology (MeSH) ; Mice, Inbred C57BL (MeSH) ; Mice, Knockout (MeSH) ; Nerve Tissue Proteins: genetics (MeSH) ; Nerve Tissue Proteins: metabolism (MeSH) ; Neurons: metabolism (MeSH) ; Neurons: physiology (MeSH) ; Photic Stimulation (MeSH) ; Receptors, AMPA: metabolism (MeSH) ; Retina: cytology (MeSH) ; Retina: metabolism (MeSH) ; Retina: physiology (MeSH) ; Synapses: physiology (MeSH) ; Synaptic Transmission: genetics (MeSH) ; Synaptic Transmission: physiology (MeSH) ; CKAMP44 protein, mouse ; Nerve Tissue Proteins ; Receptors, AMPA

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Contributing Institute(s):

1. Synaptic Signalling and Neurodegeneration (AG Engelhardt)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2018

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Database coverage:
; ; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF >= 15 ; JCR ; SCOPUS ; Web of Science Core Collection ; Zoological Record

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