TY  - JOUR
AU  - Chen, Xufeng
AU  - Aslam, Muhammad
AU  - Gollisch, Tim
AU  - Allen, Kevin
AU  - Engelhardt, Jakob
TI  - CKAMP44 modulates integration of visual inputs in the lateral geniculate nucleus.
JO  - Nature Communications
VL  - 9
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - DZNE-2020-06058
SP  - 261
PY  - 2018
AB  - Relay neurons in the dorsal lateral geniculate nucleus (dLGN) receive excitatory inputs from retinal ganglion cells (RGCs). Retinogeniculate synapses are characterized by a prominent short-term depression of AMPA receptor (AMPAR)-mediated currents, but the underlying mechanisms and its function for visual integration are not known. Here we identify CKAMP44 as a crucial auxiliary subunit of AMPARs in dLGN relay neurons, where it increases AMPAR-mediated current amplitudes and modulates gating of AMPARs. Importantly, CKAMP44 is responsible for the distinctive short-term depression in retinogeniculate synapses by reducing the rate of recovery from desensitization of AMPARs. Genetic deletion of CKAMP44 strongly reduces synaptic short-term depression, which leads to increased spike probability of relay neurons when activated with high-frequency inputs from retinogeniculate synapses. Finally, in vivo recordings reveal augmented ON- and OFF-responses of dLGN neurons in CKAMP44 knockout (CKAMP44-/-) mice, demonstrating the importance of CKAMP44 for modulating synaptic short-term depression and visual input integration.
KW  - Animals
KW  - Electroretinography
KW  - Excitatory Postsynaptic Potentials: physiology
KW  - Female
KW  - Geniculate Bodies: cytology
KW  - Geniculate Bodies: metabolism
KW  - Geniculate Bodies: physiology
KW  - Mice, Inbred C57BL
KW  - Mice, Knockout
KW  - Nerve Tissue Proteins: genetics
KW  - Nerve Tissue Proteins: metabolism
KW  - Neurons: metabolism
KW  - Neurons: physiology
KW  - Photic Stimulation
KW  - Receptors, AMPA: metabolism
KW  - Retina: cytology
KW  - Retina: metabolism
KW  - Retina: physiology
KW  - Synapses: physiology
KW  - Synaptic Transmission: genetics
KW  - Synaptic Transmission: physiology
KW  - CKAMP44 protein, mouse (NLM Chemicals)
KW  - Nerve Tissue Proteins (NLM Chemicals)
KW  - Receptors, AMPA (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:29343769
C2  - pmc:PMC5772470
DO  - DOI:10.1038/s41467-017-02415-1
UR  - https://pub.dzne.de/record/139736
ER  -