TY - JOUR
AU - Peitz, Michael
AU - Bechler, Tamara
AU - Thiele, Cornelia
AU - Veltel, Monika
AU - Bloschies, Melanie
AU - Fliessbach, Klaus
AU - Ramirez, Alfredo
AU - Brüstle, Oliver
TI - Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype.
JO - Stem cell research
VL - 29
SN - 1873-5061
CY - Amsterdam [u.a.]
PB - Elsevier
M1 - DZNE-2020-06325
SP - 250-253
PY - 2018
AB - Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele.
KW - Kruppel-Like Factor 4
KW - Aged, 80 and over
KW - Alzheimer Disease: diagnosis
KW - Alzheimer Disease: genetics
KW - Alzheimer Disease: metabolism
KW - Alzheimer Disease: pathology
KW - Apolipoprotein E4: genetics
KW - Apolipoprotein E4: metabolism
KW - Blood Cells: metabolism
KW - Blood Cells: pathology
KW - Cellular Reprogramming Techniques
KW - Genotype
KW - Humans
KW - Induced Pluripotent Stem Cells: metabolism
KW - Induced Pluripotent Stem Cells: pathology
KW - Male
KW - Polymorphism, Single Nucleotide
KW - Transcription Factors: biosynthesis
KW - Transcription Factors: genetics
KW - Apolipoprotein E4 (NLM Chemicals)
KW - Transcription Factors (NLM Chemicals)
LB - PUB:(DE-HGF)16
C6 - pmid:29753274
DO - DOI:10.1016/j.scr.2018.04.011
UR - https://pub.dzne.de/record/140003
ER -