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| Home > Publications Database > The choice of embedding media affects image quality, tissue R2* , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T. > print |
| Home > Publications Database > The choice of embedding media affects image quality, tissue R2* , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T. > print |
| 001 | 140592 | ||
| 005 | 20250417114646.0 | ||
| 024 | 7 | _ | |a 10.1002/mrm.27595 |2 doi |
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| 024 | 7 | _ | |a 0740-3194 |2 ISSN |
| 024 | 7 | _ | |a 1522-2594 |2 ISSN |
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| 037 | _ | _ | |a DZNE-2020-06914 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Dusek, Petr |0 P:(DE-HGF)0 |b 0 |e Corresponding author |
| 245 | _ | _ | |a The choice of embedding media affects image quality, tissue R2* , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T. |
| 260 | _ | _ | |a New York, NY [u.a.] |c 2019 |b Wiley-Liss |
| 264 | _ | 1 | |3 online |2 Crossref |b Wiley |c 2018-12-02 |
| 264 | _ | 1 | |3 print |2 Crossref |b Wiley |c 2019-04-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1744883161_11896 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T1 and T2* -weighted) MR images, (3) effective transverse relaxation rate (R2* ), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens.Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2 O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions.PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2 O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R2* . Imaging with agarose, D2 O, and Galden returned lower R2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R2* behaviors) for formalin and agarose.Embedding media affect gradient-echo image quality, R2* , and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2 O embedding. Formalin significantly increased R2* causing noisier data and increased QSM variance. |
| 536 | _ | _ | |a 344 - Clinical and Health Care Research (POF3-344) |0 G:(DE-HGF)POF3-344 |c POF3-344 |f POF III |x 0 |
| 542 | _ | _ | |i 2018-12-02 |2 Crossref |u http://onlinelibrary.wiley.com/termsAndConditions#vor |
| 542 | _ | _ | |i 2018-12-02 |2 Crossref |u http://doi.wiley.com/10.1002/tdm_license_1.1 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 2 | |a Aged |2 MeSH |
| 650 | _ | 2 | |a Autopsy: instrumentation |2 MeSH |
| 650 | _ | 2 | |a Autopsy: methods |2 MeSH |
| 650 | _ | 2 | |a Brain: diagnostic imaging |2 MeSH |
| 650 | _ | 2 | |a Brain: pathology |2 MeSH |
| 650 | _ | 2 | |a Brain Mapping: methods |2 MeSH |
| 650 | _ | 2 | |a Contrast Media |2 MeSH |
| 650 | _ | 2 | |a Deuterium Oxide |2 MeSH |
| 650 | _ | 2 | |a Ethers |2 MeSH |
| 650 | _ | 2 | |a Female |2 MeSH |
| 650 | _ | 2 | |a Fluorocarbons |2 MeSH |
| 650 | _ | 2 | |a Formaldehyde |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Image Processing, Computer-Assisted |2 MeSH |
| 650 | _ | 2 | |a Magnetic Resonance Imaging: instrumentation |2 MeSH |
| 650 | _ | 2 | |a Magnetic Resonance Imaging: methods |2 MeSH |
| 650 | _ | 2 | |a Male |2 MeSH |
| 650 | _ | 2 | |a Middle Aged |2 MeSH |
| 650 | _ | 2 | |a Phosphates |2 MeSH |
| 650 | _ | 2 | |a Sepharose: chemistry |2 MeSH |
| 650 | _ | 2 | |a Signal-To-Noise Ratio |2 MeSH |
| 650 | _ | 2 | |a Specimen Handling |2 MeSH |
| 650 | _ | 2 | |a Tissue Embedding: instrumentation |2 MeSH |
| 700 | 1 | _ | |a Madai, Vince Istvan |b 1 |
| 700 | 1 | _ | |a Huelnhagen, Till |b 2 |
| 700 | 1 | _ | |a Bahn, Erik |b 3 |
| 700 | 1 | _ | |a Matej, Radoslav |b 4 |
| 700 | 1 | _ | |a Sobesky, Jan |b 5 |
| 700 | 1 | _ | |a Niendorf, Thoralf |b 6 |
| 700 | 1 | _ | |a Acosta-Cabronero, Julio |0 P:(DE-2719)2810751 |b 7 |u dzne |
| 700 | 1 | _ | |a Wuerfel, Jens |b 8 |
| 773 | 1 | 8 | |a 10.1002/mrm.27595 |b : Wiley, 2018-12-02 |n 4 |p 2688-2701 |3 journal-article |2 Crossref |t Magnetic Resonance in Medicine |v 81 |y 2018 |x 0740-3194 |
| 773 | _ | _ | |a 10.1002/mrm.27595 |g Vol. 81, no. 4, p. 2688 - 2701 |0 PERI:(DE-600)1493786-4 |n 4 |q 81:4<2688 - 2701 |p 2688-2701 |t Magnetic resonance in medicine |v 81 |y 2019 |x 0740-3194 |
| 856 | 4 | _ | |u https://pub.dzne.de/record/140592/files/DZNE-2020-06914_Restricted.pdf |
| 856 | 4 | _ | |u https://pub.dzne.de/record/140592/files/DZNE-2020-06914_Restricted.pdf?subformat=pdfa |x pdfa |
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| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 7 |6 P:(DE-2719)2810751 |
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| 914 | 1 | _ | |y 2019 |
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| 920 | 1 | _ | |0 I:(DE-2719)1310001 |k AG Nestor |l Cognitive Neurology and Neurodegeneration |x 0 |
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