Journal Article

DZNE-2020-06914

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The choice of embedding media affects image quality, tissue R2* , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T.

Dusek, P. (Corresponding author) ; Madai, V. I. ; Huelnhagen, T. ; Bahn, E. ; Matej, R. ; Sobesky, J. ; Niendorf, T. ; Acosta-Cabronero, J.DZNE* ; Wuerfel, J.

2019
Wiley-Liss New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1002/mrm.27595

Abstract: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T1 and T2* -weighted) MR images, (3) effective transverse relaxation rate (R2* ), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens.Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2 O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions.PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2 O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R2* . Imaging with agarose, D2 O, and Galden returned lower R2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R2* behaviors) for formalin and agarose.Embedding media affect gradient-echo image quality, R2* , and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2 O embedding. Formalin significantly increased R2* causing noisier data and increased QSM variance.

Keyword(s): Aged (MeSH) ; Autopsy: instrumentation (MeSH) ; Autopsy: methods (MeSH) ; Brain: diagnostic imaging (MeSH) ; Brain: pathology (MeSH) ; Brain Mapping: methods (MeSH) ; Contrast Media (MeSH) ; Deuterium Oxide (MeSH) ; Ethers (MeSH) ; Female (MeSH) ; Fluorocarbons (MeSH) ; Formaldehyde (MeSH) ; Humans (MeSH) ; Image Processing, Computer-Assisted (MeSH) ; Magnetic Resonance Imaging: instrumentation (MeSH) ; Magnetic Resonance Imaging: methods (MeSH) ; Male (MeSH) ; Middle Aged (MeSH) ; Phosphates (MeSH) ; Sepharose: chemistry (MeSH) ; Signal-To-Noise Ratio (MeSH) ; Specimen Handling (MeSH) ; Tissue Embedding: instrumentation (MeSH)

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Contributing Institute(s):

1. Cognitive Neurology and Neurodegeneration (AG Nestor)

Research Program(s):

1. 344 - Clinical and Health Care Research (POF3-344) (POF3-344)

Appears in the scientific report 2019

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Web of Science Core Collection

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